The Case CCC Athymic Animal &Xenograft Core Facility includes two sites, one at CWRU and one at CCF. The Core is directed by Daniel Lindner and co-directed by Lili Liu. Case CCC members from 7 of the 8 research programs utilize the Core for their studies. In particular, the Cancer Cell Signaling, GU Malignancies, Hematopoietic Disorders, and Developmental Therapeutics Programs utilize the Core. The Core provides a facility for breeding and housing of athymic nude mice (NCRnu/nu), NOD-SCID mice, and other immunodeficient animals. The Core also provides pathogen-free facilities for experimentation using immunodeficient animal hosts and xenografts of human cell lines, human tumors, and normal human cells, in particular hematopoietic cells. The Core helps investigators design and perform experiments, tumor measurements, draw blood samples, and monitoring functions. Core staff maintain and monitor animals housed in the facility, and provide users with many services that facilitate research projects including transportation from one site to another, transportation to the animal imaging and radiation facilities, health reports, animal handling, and animal handling training. For individual investigator research, the Core teaches experimental techniques and performs experimental procedures. Investigators use mice in the facility for studies of human tumor xenografts to test anticancer drug effects on syngeneic murine tumor implants, to determine species-specific effect of cytokines and evaluate cytotoxic T cell (CTL) response to tumor associated antigens;to test oncogenicity of specific tumor genes on human tumor xenografts;and to perform small animal imaging to detect cancer size and location, as well as anticancer drug distribution and pharmacokinefic study in vivo. The Core has provided key support for studies investigating combination treatment of the protein tyrosine phosphatase (PTPase) inhibitors with agents that sensitize inhibitor resistant tumors in mouse models. Mouse tumor studies performed by the Core were pivotal to delineate the role and mechanism of action of oncogenic PTPases in malignant diseases. In addition. Xenograft studies were critical to study the growth inhibitory effects of apigenin;to determine whether pharmacologic intervention with apigenin has a direct growth inhibitory effect on human prostate tumors implanted in athymic nude mice;and to examine cell cycle regulatory molecules as precise molecular targets of apigenin action.
The Case Comprehensive Cancer Center is Northeast Ohio's only NCI designated comprehensive cancer center providing bench-to-bedside medical research involving partnerships between basic, clinical and population scientists to speed translation of laboratory discoveries into new prevention/intervention and cancer treatments.
|Brown, Amanda L; Mark Brown, J (2017) Critical roles for ?/? hydrolase domain 5 (ABHD5)/comparative gene identification-58 (CGI-58) at the lipid droplet interface and beyond. Biochim Biophys Acta 1862:1233-1241|
|Thiagarajan, Praveena S; Zheng, Qiao; Bhagrath, Manvir et al. (2017) STAT3 activation by leptin receptor is essential for TNBC stem cell maintenance. Endocr Relat Cancer 24:415-426|
|Yao, Peng; Wu, Jiangbin; Lindner, Daniel et al. (2017) Interplay between miR-574-3p and hnRNP L regulates VEGFA mRNA translation and tumorigenesis. Nucleic Acids Res 45:7950-7964|
|Donnola, Shannon B; Dasenbrook, Elliott C; Weaver, David et al. (2017) Preliminary comparison of normalized T1 and non-contrast perfusion MRI assessments of regional lung disease in cystic fibrosis patients. J Cyst Fibros 16:283-290|
|Doherty, Mary R; Cheon, HyeonJoo; Junk, Damian J et al. (2017) Interferon-beta represses cancer stem cell properties in triple-negative breast cancer. Proc Natl Acad Sci U S A 114:13792-13797|
|Saygin, Caner; Wiechert, Andrew; Rao, Vinay S et al. (2017) CD55 regulates self-renewal and cisplatin resistance in endometrioid tumors. J Exp Med 214:2715-2732|
|Samaeekia, Ravand; Adorno-Cruz, Valery; Bockhorn, Jessica et al. (2017) miR-206 Inhibits Stemness and Metastasis of Breast Cancer by Targeting MKL1/IL11 Pathway. Clin Cancer Res 23:1091-1103|
|Flocke, Susan A; Hoffman, Richard; Eberth, Jan M et al. (2017) The Prevalence of Tobacco Use at Federally Qualified Health Centers in the United States, 2013. Prev Chronic Dis 14:E29|
|Balanis, Nikolas; Carlin, Cathleen R (2017) Stress-induced EGF receptor signaling through STAT3 and tumor progression in triple-negative breast cancer. Mol Cell Endocrinol 451:24-30|
|Johansen, Mette L; Gao, Ying; Hutnick, Melanie A et al. (2017) Quantitative Molecular Imaging with a Single Gd-Based Contrast Agent Reveals Specific Tumor Binding and Retention in Vivo. Anal Chem 89:5932-5939|
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