The Case CCC Athymic Animal &Xenograft Core Facility includes two sites, one at CWRU and one at CCF. The Core is directed by Daniel Lindner and co-directed by Lili Liu. Case CCC members from 7 of the 8 research programs utilize the Core for their studies. In particular, the Cancer Cell Signaling, GU Malignancies, Hematopoietic Disorders, and Developmental Therapeutics Programs utilize the Core. The Core provides a facility for breeding and housing of athymic nude mice (NCRnu/nu), NOD-SCID mice, and other immunodeficient animals. The Core also provides pathogen-free facilities for experimentation using immunodeficient animal hosts and xenografts of human cell lines, human tumors, and normal human cells, in particular hematopoietic cells. The Core helps investigators design and perform experiments, tumor measurements, draw blood samples, and monitoring functions. Core staff maintain and monitor animals housed in the facility, and provide users with many services that facilitate research projects including transportation from one site to another, transportation to the animal imaging and radiation facilities, health reports, animal handling, and animal handling training. For individual investigator research, the Core teaches experimental techniques and performs experimental procedures. Investigators use mice in the facility for studies of human tumor xenografts to test anticancer drug effects on syngeneic murine tumor implants, to determine species-specific effect of cytokines and evaluate cytotoxic T cell (CTL) response to tumor associated antigens;to test oncogenicity of specific tumor genes on human tumor xenografts;and to perform small animal imaging to detect cancer size and location, as well as anticancer drug distribution and pharmacokinefic study in vivo. The Core has provided key support for studies investigating combination treatment of the protein tyrosine phosphatase (PTPase) inhibitors with agents that sensitize inhibitor resistant tumors in mouse models. Mouse tumor studies performed by the Core were pivotal to delineate the role and mechanism of action of oncogenic PTPases in malignant diseases. In addition. Xenograft studies were critical to study the growth inhibitory effects of apigenin;to determine whether pharmacologic intervention with apigenin has a direct growth inhibitory effect on human prostate tumors implanted in athymic nude mice;and to examine cell cycle regulatory molecules as precise molecular targets of apigenin action.
The Case Comprehensive Cancer Center is Northeast Ohio's only NCI designated comprehensive cancer center providing bench-to-bedside medical research involving partnerships between basic, clinical and population scientists to speed translation of laboratory discoveries into new prevention/intervention and cancer treatments.
|Anderson, Christian E; Wang, Charlie Y; Gu, Yuning et al. (2018) Regularly incremented phase encoding - MR fingerprinting (RIPE-MRF) for enhanced motion artifact suppression in preclinical cartesian MR fingerprinting. Magn Reson Med 79:2176-2182|
|Burger, Denis R; Parker, Yvonne; Guinta, Kathryn et al. (2018) PRO 140 Monoclonal Antibody to CCR5 Prevents Acute Xenogeneic Graft-versus-Host Disease in NOD-scid IL-2Rynull Mice. Biol Blood Marrow Transplant 24:260-266|
|Shi, Xiaojun; Wang, Bingcheng (2018) Caught in the ""Akt"": Cross-talk between EphA2 and EGFR through the Akt-PIKfyve axis maintains cellular sensitivity to EGF. Sci Signal 11:|
|Tartakoff, Alan Michael; Dulce, David; Landis, Elizabeth (2018) Delayed Encounter of Parental Genomes Can Lead to Aneuploidy in Saccharomyces cerevisiae. Genetics 208:139-151|
|Gromovsky, Anthony D; Schugar, Rebecca C; Brown, Amanda L et al. (2018) ?-5 Fatty Acid Desaturase FADS1 Impacts Metabolic Disease by Balancing Proinflammatory and Proresolving Lipid Mediators. Arterioscler Thromb Vasc Biol 38:218-231|
|Ignatz-Hoover, James J; Wang, Victoria; Mackowski, Nathan M et al. (2018) Aberrant GSK3? nuclear localization promotes AML growth and drug resistance. Blood Adv 2:2890-2903|
|Hubler, Zita; Allimuthu, Dharmaraja; Bederman, Ilya et al. (2018) Accumulation of 8,9-unsaturated sterols drives oligodendrocyte formation and remyelination. Nature 560:372-376|
|Asthana, Abhishek; Ramakrishnan, Parameswaran; Vicioso, Yorleny et al. (2018) Hexosamine Biosynthetic Pathway Inhibition Leads to AML Cell Differentiation and Cell Death. Mol Cancer Ther 17:2226-2237|
|Belur Nagaraj, Anil; Kovalenko, Olga; Avelar, Rita et al. (2018) Mitotic Exit Dysfunction through the Deregulation of APC/C Characterizes Cisplatin-Resistant State in Epithelial Ovarian Cancer. Clin Cancer Res 24:4588-4601|
|Yang, Xiaosong; Pan, You; Qiu, Zhaojun et al. (2018) RNF126 as a Biomarker of a Poor Prognosis in Invasive Breast Cancer and CHEK1 Inhibitor Efficacy in Breast Cancer Cells. Clin Cancer Res 24:1629-1643|
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