The Macromolecular Structure Shared Resource (MSSR) is comprised of the X-ray crystallography (X-ray) and the Nuclear Magnetic Resonance (NMR) laboratories. X-ray crystallography and NMR are highly complementary methods for elucidating three-dimensional structures and for studying macromolecular interactions. Together, they provide Cancer Center members with comprehensive methodologies to understand how cancer-related biological macromolecules function in normal and diseased states at the molecular level. Cancer Center investigators will be advised and assisted in utilizing these sophisticated technologies to determine structures of cancer-related biological macromolecules and to investigate their interactions with other macromolecules and with potential therapeutic agents by the Directors of the X-ray and NMR laboratories, P. John Hart, PhD and Andrew P. Hinck, PhD, respectively. The X-ray component of the MSSR includes an Art Robbins crystallization robot and two state-of-the-art Rigaku-MSSR X-ray data collection systems. The instrumentation provides full capabilities for conducting all modern X-ray diffraction experiments and is suitable for obtaining high quality three-dimensional structures of proteins, nucleic acids and their complexes. The NMR component of the MSSR includes state-of-the-art Bruker spectrometers equipped with high sensitivity cryoprobes operating at 500, 600, and 700 MHz. The instrumentation provides full capabilities for conducting modern NMR experiments with N, C, and H labeled macromolecules and is suitable for obtaining three-dimensional solution structures and investigating interactions with other macromolecules and potential therapeutic agents. The MSSR is made accessible to the broader Cancer Center Membership by PhD-trained technical managers, Alex Taylor, PhD, and Udayar llangovan, PhD, for the X-ray and NMR laboratories, respectively, who provide guidance at each step in the process, from sample preparation to interpretation and presentation of results. The MSSR provides a comprehensive array of methodologies with which to visualize and functionally characterize cancer-related biological macromolecules.

Public Health Relevance

The key to determining the function of a molecule and how it is altered by mutation is facilitated by elucidating the higher level structure. The Macromolecular Structure Shared Resource provides NMR and Xray crystallography capabilities to cancer center members. This information obtained by this shared resource provides essential information not only for basic research, but also for translational applications.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
3P30CA054174-19S5
Application #
8637195
Study Section
Subcommittee G - Education (NCI)
Project Start
1997-08-01
Project End
2014-07-31
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
19
Fiscal Year
2013
Total Cost
$34,172
Indirect Cost
$11,314
Name
University of Texas Health Science Center San Antonio
Department
Type
DUNS #
800772162
City
San Antonio
State
TX
Country
United States
Zip Code
78229
Ghosh, Sagar; Hughes, Daniel; Parma, Dorothy Long et al. (2014) Association of obesity and circulating adipose stromal cells among breast cancer survivors. Mol Biol Rep 41:2907-16
Meng, Jia; Lu, Zhiliang; Liu, Hui et al. (2014) A protocol for RNA methylation differential analysis with MeRIP-Seq data and exomePeak R/Bioconductor package. Methods 69:274-81
Gong, Jingjing; Muñoz, Amanda R; Chan, Daniel et al. (2014) STAT3 down regulates LC3 to inhibit autophagy and pancreatic cancer cell growth. Oncotarget 5:2529-41
Fok, Wilson C; Livi, Carolina; Bokov, Alex et al. (2014) Short-term rapamycin treatment in mice has few effects on the transcriptome of white adipose tissue compared to dietary restriction. Mech Ageing Dev 140:23-9
Morales, Liza D; Casillas Pavón, Edgar A; Shin, Jun Wan et al. (2014) Protein tyrosine phosphatases PTP-1B, SHP-2, and PTEN facilitate Rb/E2F-associated apoptotic signaling. PLoS One 9:e97104
Mousavi, Seyed Mohsen; Sundquist, Jan; Hemminki, Kari (2014) Risk of Kaposi sarcoma among immigrants to Sweden. Acta Derm Venereol 94:476-7
Ankerst, Donna P; Boeck, Andreas; Freedland, Stephen J et al. (2014) Evaluating the Prostate Cancer Prevention Trial High Grade Prostate Cancer Risk Calculator in 10 international biopsy cohorts: results from the Prostate Biopsy Collaborative Group. World J Urol 32:185-91
Biswas, Tanuka; Gu, Xiang; Yang, Junhua et al. (2014) Attenuation of TGF-* signaling supports tumor progression of a mesenchymal-like mammary tumor cell line in a syngeneic murine model. Cancer Lett 346:129-38
Ramirez, Amelie G; Munoz, Edgar; Holden, Alan E C et al. (2014) Incidence of hepatocellular carcinoma in Texas Latinos, 1995-2010: an update. PLoS One 9:e99365
Bansal, H; Yihua, Q; Iyer, S P et al. (2014) WTAP is a novel oncogenic protein in acute myeloid leukemia. Leukemia 28:1171-4

Showing the most recent 10 out of 616 publications