Abstract

9.4.1 ABSTRACT: FLOW CYTOMETRY Director: Tucker LeBien, Ph.D. The mission of the Cancer Center Flow Cytometry Core (hereafter referred to as the Flow Core) is to provide state-of-the-art flow cytometry/cell sorting services, scientific consultation, and technical support to facilitate interaction and advance scientific discovery in a reliable and cost-effective manner. The Flow Core has a 3- laser FACSVantage SE (equipped for high-speed sorting and upgraded with the DiVa option), a 3-laser FACSAria, a 3-laser LSR (I, four FACSCalibur benchtop cytometers and an Amnis ImageStream 100 imaging scanning cytometer. The major services offered are: 1) high-speed multiparameter cell sorting (conducted by a full-time dedicated operator), b) individual investigator training and access to the FACSCaliburs and LSR II, and 3) individual or small group consultation on data acquisition and analysis. The success of the Flow Core is evident by the quality of the science it supports and the broad range of users encompassing the seven research programs.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA077598-15
Application #
8449997
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2013-02-01
Budget End
2014-01-31
Support Year
15
Fiscal Year
2013
Total Cost
$118,265
Indirect Cost
$39,943

  Institution

Name
University of Minnesota Twin Cities
Department
Type
DUNS #
555917996
City
Minneapolis
State
MN
Country
United States
Zip Code
55455

  Publications

Boatman, Jeffrey A; Vock, David M; Koopmeiners, Joseph S et al. (2018) Estimating causal effects from a randomized clinical trial when noncompliance is measured with error. Biostatistics 19:103-118
Guo, Jingshu; Villalta, Peter W; Weight, Christopher J et al. (2018) Targeted and Untargeted Detection of DNA Adducts of Aromatic Amine Carcinogens in Human Bladder by Ultra-Performance Liquid Chromatography-High-Resolution Mass Spectrometry. Chem Res Toxicol :
Rashidi, Armin; Shanley, Ryan; Yohe, Sophia L et al. (2018) Recipient single nucleotide polymorphisms in Paneth cell antimicrobial peptide genes and acute graft-versus-host disease: analysis of BMT CTN-0201 and -0901 samples. Br J Haematol 182:887-894
Teitelbaum, A M; Murphy, S E; Akk, G et al. (2018) Nicotine dependence is associated with functional variation in FMO3, an enzyme that metabolizes nicotine in the brain. Pharmacogenomics J 18:136-143
Murphy, Sharon E; von Weymarn, Linda B; Parenteau, Marc et al. (2018) Influence of UGT2B10 Genotype on Urinary Excretion of 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol- N-glucuronide by African American Smokers. Chem Res Toxicol 31:168-175
Bellamri, Medjda; Xiao, Shun; Murugan, Paari et al. (2018) Metabolic Activation of the Cooked Meat Carcinogen 2-Amino-1-Methyl-6-Phenylimidazo[4,5-b]Pyridine in Human Prostate. Toxicol Sci 163:543-556
Jarvis, Matthew C; Ebrahimi, Diako; Temiz, Nuri A et al. (2018) Mutation Signatures Including APOBEC in Cancer Cell Lines. JNCI Cancer Spectr 2:
Hegerova, Livia; Bachan, Adam; Cao, Qing et al. (2018) Catheter-Related Thrombosis in Patients with Lymphoma or Myeloma Undergoing Autologous Stem Cell Transplantation. Biol Blood Marrow Transplant 24:e20-e25
Lassig, Amy Anne D; Bechtold, Joan E; Lindgren, Bruce R et al. (2018) Tobacco exposure and wound healing in head and neck surgical wounds. Laryngoscope 128:618-625
Petzel, Sue V; Isaksson Vogel, Rachel; Cragg, Julie et al. (2018) Effects of web-based instruction and patient preferences on patient-reported outcomes and learning for women with advanced ovarian cancer: A randomized controlled trial. J Psychosoc Oncol 36:503-519

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