The Flow Cytometry shared facility has been in existence since 1985 and has provided essential flow cytometric services to many Cancer Center members from all research programs of the Center. The mission of this facility has been to serve the varied research needs of researchers at the ID Simon Cancer Center. Our goal is to continue to provide Cancer Center members access to cost-effective state-of-the-art technology. The fact that during the current funding period on average more than one-half of all IUSCC full and associate members used the facility in a given year is testimony to its importance. Flow Cytometry activities are currently carried out by three full-time employees, all of whom are well-qualified operators that have received extensive training. The facility provides consultation, technical advice, and collaboration. We believe that a centralized facility providing these services promotes cutting edge science and also serves as a central common area for Cancer Center investigators to interact and exchange scientific information. Since the inception of this facility, there have been many requests for new services. As new techniques were added, the list of services provided by the facility has grown. The personnel and instrumentation available at the facility are capable of performing virtually any flow cytometric assay requested by Cancer Center investigators. The list of available services thus includes: 1. Multiparameter (up to 9- and 10-color) immunofluorescence analysis and cell sorting, respectively 2. DMA content and cell cycle analysis 3. Simultaneous immunofluorescence and cell cycle analysis 4. Sorting of single cells and small number of cells into different types of plates/flasks 5. Cell sorting (bulk or single cells) based on position of cells in cell cycle 6. Apoptosis analysis 7. BrdU incorporation 8. Viability assays 9. Chromosome analysis (univariate and bivariate) 10. Receptor - ligand interactions 11. Kinetic analyses 12. Calcium influx analysis 13. Off-line data analysis

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National Cancer Institute (NCI)
Center Core Grants (P30)
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Subcommittee G - Education (NCI)
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Indiana University-Purdue University at Indianapolis
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Cohee, Andrea A; Adams, Rebecca N; Johns, Shelley A et al. (2017) Long-term fear of recurrence in young breast cancer survivors and partners. Psychooncology 26:22-28
Wang, Haiyan; Cai, Shanbao; Bailey, Barbara J et al. (2017) Combination therapy in a xenograft model of glioblastoma: enhancement of the antitumor activity of temozolomide by an MDM2 antagonist. J Neurosurg 126:446-459
Caballero, Sergio; Kent, David L; Sengupta, Nilanjana et al. (2017) Bone Marrow-Derived Cell Recruitment to the Neurosensory Retina and Retinal Pigment Epithelial Cell Layer Following Subthreshold Retinal Phototherapy. Invest Ophthalmol Vis Sci 58:5164-5176
Salazar, Tatiana E; Richardson, Matthew R; Beli, Eleni et al. (2017) Electroacupuncture Promotes Central Nervous System-Dependent Release of Mesenchymal Stem Cells. Stem Cells 35:1303-1315
Kedage, Vivekananda; Strittmatter, Brady G; Dausinas, Paige B et al. (2017) Phosphorylation of the oncogenic transcription factor ERG in prostate cells dissociates polycomb repressive complex 2, allowing target gene activation. J Biol Chem 292:17225-17235
Munnamalai, Vidhya; Sienknecht, Ulrike J; Duncan, R Keith et al. (2017) Wnt9a Can Influence Cell Fates and Neural Connectivity across the Radial Axis of the Developing Cochlea. J Neurosci 37:8975-8988
Hernandez-Perez, Marimar; Chopra, Gaurav; Fine, Jonathan et al. (2017) Inhibition of 12/15-Lipoxygenase Protects Against ?-Cell Oxidative Stress and Glycemic Deterioration in Mouse Models of Type 1 Diabetes. Diabetes 66:2875-2887
Hahn, Noah M; Bivalacqua, Trinity J; Ross, Ashley E et al. (2017) A Phase II Trial of Dovitinib in BCG-Unresponsive Urothelial Carcinoma with FGFR3 Mutations or Overexpression: Hoosier Cancer Research Network Trial HCRN 12-157. Clin Cancer Res 23:3003-3011
Mohamad, Safa F; Xu, Linlin; Ghosh, Joydeep et al. (2017) Osteomacs interact with megakaryocytes and osteoblasts to regulate murine hematopoietic stem cell function. Blood Adv 1:2520-2528
Shah, Fenil; Goossens, Emery; Atallah, Nadia M et al. (2017) APE1/Ref-1 knockdown in pancreatic ductal adenocarcinoma - characterizing gene expression changes and identifying novel pathways using single-cell RNA sequencing. Mol Oncol 11:1711-1732

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