The Siteman Cancer Center Molecular and Genomic Analysis (MGA) Core represents the physical and programmatic integration of two previous cores, the Multiplexed Gene Analysis (GeneChip) Core and the Molecular Core. The unified vision and specific aims of this core facility are: 1) To provide members with facilitated and cost-effective access to high complexity genomic analysis platforms including Affymetrix GeneChipd) microarray technology for whole genome analysis of gene transcription, genotyping, genome copy number, and DNA sequence, as well as PCR-based directed DNA resequencing and quantitative PCR instrumentation, and;2) To provide a clinically accredited laboratory environment for developing, validating, and translating genome-based biomarker assays toward their use in clinical trials and ultimately, routine patient management. To accomplish these aims, the Core continues to provide Affymetrix microarray services to investigators. All array platforms are supported for human and many other model organism species, including custom genotyping arrays that implement four-color, multiplexed genotyping assays using Molecular Inversion Probe (MIP) chemistry. New RNA amplification protocols have been validated for expression profiling from limiting amounts of clinical material (such as flow sorted cell populations or microdissected tissue) as well paraffin embedded tissues. A reorganization of operations and the conduct of CLIA-Iicensed clinical testing have promoted a new level of quality control and proficiency testing associated with assays conducted even for purely research purposes. New staff and additional faculty oversight also allow for consultation with investigators for 'CLIA-grade'assay development and interpretation in the context of clinical and translational studies. Like its predecessors, the new MGA Core maintains increasingly close integration with the Tissue Procurement Core and the Bioinformatics Core, to provide a seamless conduit for the collection, analysis, and interpretation of genomic data generated from clinical and experimental biospecimens.
The Siteman Cancer Center Molecular and Genomic Analysis (MGA) Core allows cancer scientists to perform complex and expensive genome studies that would not othen/vise be possible in their own laboratories. The data generated by use of this facility is helping researchers to better understand the genetic basis of cancer which, in turn, will lead to new approaches for cancer treatment and prevention.
|Engle, E K; Fisher, D A C; Miller, C A et al. (2015) Clonal evolution revealed by whole genome sequencing in a case of primary myelofibrosis transformed to secondary acute myeloid leukemia. Leukemia 29:869-76|
|Zihni, Ahmed M; Cavallo, Jaime A; Thompson Jr, Dominic M et al. (2015) Evaluation of absorbable mesh fixation devices at various deployment angles. Surg Endosc 29:1605-13|
|Rosenbaum, Joan L; Smith, Joan R; Yan, Yan et al. (2015) Impact of a Neonatal-Bereavement-Support DVD on Parental Grief: A Randomized Controlled Trial. Death Stud 39:191-200|
|Solga, Anne C; Pong, Winnie W; Walker, Jason et al. (2015) RNA-sequencing reveals oligodendrocyte and neuronal transcripts in microglia relevant to central nervous system disease. Glia 63:531-48|
|Yamada, Tomoko; Yang, Yue; Hemberg, Martin et al. (2014) Promoter decommissioning by the NuRD chromatin remodeling complex triggers synaptic connectivity in the mammalian brain. Neuron 83:122-34|
|Lewis Jr, James S; Ali, Sahirzeeshan; Luo, Jingqin et al. (2014) A quantitative histomorphometric classifier (QuHbIC) identifies aggressive versus indolent p16-positive oropharyngeal squamous cell carcinoma. Am J Surg Pathol 38:128-37|
|Griffey, Richard T; Jeffe, Donna B; Bailey, Thomas (2014) Emergency physicians' attitudes and preferences regarding computed tomography, radiation exposure, and imaging decision support. Acad Emerg Med 21:768-77|
|Jorns, Julie M; Thomas, Dafydd G; Healy, Patrick N et al. (2014) Estrogen receptor expression is high but is of lower intensity in tubular carcinoma than in well-differentiated invasive ductal carcinoma. Arch Pathol Lab Med 138:1507-13|
|Jeffe, Donna B; Andriole, Dorothy A; Wathington, Heather D et al. (2014) Educational outcomes for students enrolled in MD-PhD programs at medical school matriculation, 1995-2000: a national cohort study. Acad Med 89:84-93|
|Tait, Sarah; Pacheco, Jose M; Gao, Feng et al. (2014) Body mass index, diabetes, and triple-negative breast cancer prognosis. Breast Cancer Res Treat 146:189-97|
Showing the most recent 10 out of 514 publications