The Hormone Assay and Analytical Services Core (HAASC) provides assistance to investigators in the measurement of hormones, amino acids ( concentration and specific activity), glucose enrichment, lipids, nucleotides, and markers of oxidative stress in biologic fluids and tissue samples. The core provides space, equipment and personnel that performs sample analysis and method development. Investigators pay a fee for service that covers the cost of regents, supplies, a percentage of personnel salary and pro-rated service contracts. Over the last grant cycle this core has dramatically evolved. With support from the institution the core has purchased equipment that will lower overall cost and decrease turnaround time for our standard high throughput assays and are continuing to offer cost effective new hormone assays to our investigators. The core has developed NMR assays to assess the enrichment of glucose (C2/C5), which is a marker of gluconeogenesis. We have expanded the scope of our services as the needs of VDRTC members change. The core assayed over 30,000 samples in the past year for 32 Vanderbilt investigators and 7 non-Vanderbilt investigators. Over the past grant cycle it provided data to support over 150 publications. The HAASC is jointly supported by the VDRTC and the NIDDK-funded Mouse Metabolic Phenotyping Center. This cooperative arrangement allows the core to offer a wide range of services in a non-overlapping, cost efficient manner. The core is part of the Vanderbilt Core Ordering &Reporting Enterprise Systemtm, which provides an efficient billing system and oversight and governance for the core. The Hormone Assay and Analytical Services Core, in operation for more than 30 years, continues to provide essential services that support the research of DRTC-affiliated investigators in the next funding cycle.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZDK1-GRB-S (J1))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Vanderbilt University Medical Center
United States
Zip Code
Gamboa, Alfredo; Okamoto, Luis E; Arnold, Amy C et al. (2014) Autonomic blockade improves insulin sensitivity in obese subjects. Hypertension 64:867-74
Kraft, Lewis J; Nguyen, Tuan A; Vogel, Steven S et al. (2014) Size, stoichiometry, and organization of soluble LC3-associated complexes. Autophagy 10:861-77
Flavin, Stephanie A; Matthews, Robert T; Wang, Qin et al. (2014) ?(2A)-adrenergic receptors filter parabrachial inputs to the bed nucleus of the stria terminalis. J Neurosci 34:9319-31
Dadi, Prasanna K; Vierra, Nicholas C; Ustione, Alessandro et al. (2014) Inhibition of pancreatic ?-cell Ca2+/calmodulin-dependent protein kinase II reduces glucose-stimulated calcium influx and insulin secretion, impairing glucose tolerance. J Biol Chem 289:12435-45
Allen, Ryan M; Vickers, Kasey C (2014) Coenzyme Q10 increases cholesterol efflux and inhibits atherosclerosis through microRNAs. Arterioscler Thromb Vasc Biol 34:1795-7
Freeman, Megan Culler; Graham, Rachel L; Lu, Xiaotao et al. (2014) Coronavirus replicase-reporter fusions provide quantitative analysis of replication and replication complex formation. J Virol 88:5319-27
Weitkamp, Jörn-Hendrik; Rosen, Michael J; Zhao, Zhiguo et al. (2014) Small intestinal intraepithelial TCR??+ T lymphocytes are present in the premature intestine but selectively reduced in surgical necrotizing enterocolitis. PLoS One 9:e99042
Limkunakul, Chutatip; Sundell, Mary B; Pouliot, Brianna et al. (2014) Glycemic load is associated with oxidative stress among prevalent maintenance hemodialysis patients. Nephrol Dial Transplant 29:1047-53
Wu, Shu-Yu; de Borsetti, Nancy Hernandez; Bain, Emily J et al. (2014) Mediator subunit 12 coordinates intrinsic and extrinsic control of epithalamic development. Dev Biol 385:13-22
Short, Kurt W; Head, W Steve; Piston, David W (2014) Connexin 36 mediates blood cell flow in mouse pancreatic islets. Am J Physiol Endocrinol Metab 306:E324-31

Showing the most recent 10 out of 139 publications