Abstract

The Epithelial Cell and Mucosal Immunology Core (Core C) provides the infrastructure, biologic resources, expertise, and training for innovative and cross-disciplinary research on epithelial biology and immune function in mucosal surfaces and solid organs of the GI tract. Specific activities and services include: Polarized Epithelial Cell Culture and Genetic Transformations nCounter NanoString RNA Analysis FACs 7- or 12-Color Analysis Of Cultured Cells or Tissue/Organoid-derived Primary Cells Transepithelial Electrophysiology of live cells, cell monolayers, and tissues Gastrointestinal Organoid Derivation and Culture Specialized Equipment, Reagents, Training, and Consultation for recombinant protein expression and purification, including small molecules, specialized and unique epithelial cell lines, expression profiling using Bio-Plex MAGPIX multiplex reader Research and Development of live cell ratio fluorescence imaging of ion and solute transients, and FRAP analysis of membrane molecules A total of 27 HDDC members and 20 non-Members used Core C in the last grant cycle as measured in billable hours of service. 8 HDDC Members used Core C services in every quarter. The Core recorded >4000 billable hours/year. 45 Members and Associate Members anticipate use of Core C in the next grant cycle. All services will be utilized.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK034854-34
Application #
9605283
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2018-12-01
Budget End
2019-11-30
Support Year
34
Fiscal Year
2019
Total Cost
Indirect Cost

  Institution

Name
Boston Children's Hospital
Department
Type
DUNS #
076593722
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Rankin, Carl Robert; Theodorou, Evangelos; Man Law, Ivy Ka et al. (2018) Identification of novel mRNAs and lncRNAs associated with mouse experimental colitis and human inflammatory bowel disease. Am J Physiol Gastrointest Liver Physiol 315:G722-G733
Richmond, Camilla A; Rickner, Hannah; Shah, Manasvi S et al. (2018) JAK/STAT-1 Signaling Is Required for Reserve Intestinal Stem Cell Activation during Intestinal Regeneration Following Acute Inflammation. Stem Cell Reports 10:17-26
Richardson, Gavin David; Sage, Andrew; Bennaceur, Karim et al. (2018) Telomerase Mediates Lymphocyte Proliferation but Not the Atherosclerosis-Suppressive Potential of Regulatory T-Cells. Arterioscler Thromb Vasc Biol 38:1283-1296
Garcia-Castillo, Maria Daniela; Lencer, Wayne I; Chinnapen, Daniel J-F (2018) Transcytosis Assay for Transport of Glycosphingolipids across MDCK-II Cells. Bio Protoc 8:
Aden, Konrad; Tran, Florian; Ito, Go et al. (2018) ATG16L1 orchestrates interleukin-22 signaling in the intestinal epithelium via cGAS-STING. J Exp Med 215:2868-2886
Hong, Shangyu; Song, Wei; Zushin, Peter-James H et al. (2018) Phosphorylation of Beta-3 adrenergic receptor at serine 247 by ERK MAP kinase drives lipolysis in obese adipocytes. Mol Metab 12:25-38
Sallis, Benjamin F; Acar, Utkucan; Hawthorne, Kelsey et al. (2018) A Distinct Esophageal mRNA Pattern Identifies Eosinophilic Esophagitis Patients With Food Impactions. Front Immunol 9:2059
Smillie, Christopher S; Sauk, Jenny; Gevers, Dirk et al. (2018) Strain Tracking Reveals the Determinants of Bacterial Engraftment in the Human Gut Following Fecal Microbiota Transplantation. Cell Host Microbe 23:229-240.e5
Li, Yang; Fu, Tian-Min; Lu, Alvin et al. (2018) Cryo-EM structures of ASC and NLRC4 CARD filaments reveal a unified mechanism of nucleation and activation of caspase-1. Proc Natl Acad Sci U S A 115:10845-10852
Li, Katherine; Strauss, Richard; Ouahed, Jodie et al. (2018) Molecular Comparison of Adult and Pediatric Ulcerative Colitis Indicates Broad Similarity of Molecular Pathways in Disease Tissue. J Pediatr Gastroenterol Nutr 67:45-52

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