Statement of work The Broad Institute will support the work of BADERC investigators through engagement of the following Broad Platforms: Metabolite Profiling, Proteomics, RNAi and Genetic Analysis. We propose two modes in which BADERC investigators can utilize this support: First is through the pre-existing Pilot and Feasibility process. We envision that a number of these P&F proposals will aim to incorporate Broad platform capabilities in their proposals (for example, a project that performs metabolic profiling and / or RNAi screening capabilities, or that utilizes SNP genotyping in a human genetic aims with the Genetic Analysis Platform). The Project Manager (Ms. Burtt) and PI (Altshuler) will help investigators identify and engage these opportunities, to interact with platform staff to assess feasibility and details of experimental design, and to write proposals utilizing these platforms. If these P&F proposals are judged meritorious and selected for funding (by the existing BADERC mechanism), then the costs incurred by the Broad platforms would be supported by the budgeted funds. The program manager would then facilitate the execution of the approved project activities. Second, there are a number of platform capabilities that will be available to BADERC investigators as needed based on their existing research. Examples might include SNP genotyping for an ongoing project that tests whether a novel candidate gene contributes to a diabetes-related phenotype in humans, or creation of new RNAi reagents to perform knock-down assays in a cellular diabetes model. The Broad subcontract will support access to such Broad platform capabilities by each BADERC investigator (up to a pre-specified limit per investigator, not to exceed the total budgeted). As above, the project manager would serve as a key liaison between BADERC investigators and Broad Platforms, helping identify which capabilities matched each funding mechanism, guiding investigators to the relevant staff, and facilitating successful execution of each approved activity.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK057521-14
Application #
8473854
Study Section
Special Emphasis Panel (ZDK1-GRB-2)
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
14
Fiscal Year
2013
Total Cost
$113,027
Indirect Cost
$16,053
Name
Massachusetts General Hospital
Department
Type
DUNS #
073130411
City
Boston
State
MA
Country
United States
Zip Code
02199
Krashes, Michael J; Shah, Bhavik P; Madara, Joseph C et al. (2014) An excitatory paraventricular nucleus to AgRP neuron circuit that drives hunger. Nature 507:238-42
Nomura, Naohiro; Nunes, Paula; Bouley, Richard et al. (2014) High-throughput chemical screening identifies AG-490 as a stimulator of aquaporin 2 membrane expression and urine concentration. Am J Physiol Cell Physiol 307:C597-605
Lee, Seung-Hwan; Huang, Hu; Choi, Kangduk et al. (2014) ROCK1 isoform-specific deletion reveals a role for diet-induced insulin resistance. Am J Physiol Endocrinol Metab 306:E332-43
Yuen, Grace J; Ausubel, Frederick M (2014) Enterococcus infection biology: lessons from invertebrate host models. J Microbiol 52:200-10
Chiappini, Franck; Catalano, Karyn J; Lee, Jennifer et al. (2014) Ventromedial hypothalamus-specific Ptpn1 deletion exacerbates diet-induced obesity in female mice. J Clin Invest 124:3781-92
Cohen, Paul; Levy, Julia D; Zhang, Yingying et al. (2014) Ablation of PRDM16 and beige adipose causes metabolic dysfunction and a subcutaneous to visceral fat switch. Cell 156:304-16
Wei, Nancy; Pan, Jessica; Pop-Busui, Rodica et al. (2014) Altered sphingoid base profiles in type 1 compared to type 2 diabetes. Lipids Health Dis 13:161
Kraus, Daniel; Yang, Qin; Kong, Dong et al. (2014) Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity. Nature 508:258-62
Oshiro, Noriko; Rapley, Joseph; Avruch, Joseph (2014) Amino acids activate mammalian target of rapamycin (mTOR) complex 1 without changing Rag GTPase guanyl nucleotide charging. J Biol Chem 289:2658-74
Ruan, Ye Chun; Wang, Yan; Da Silva, Nicolas et al. (2014) CFTR interacts with ZO-1 to regulate tight junction assembly and epithelial differentiation through the ZONAB pathway. J Cell Sci 127:4396-408

Showing the most recent 10 out of 209 publications