Core CThe Developmental Neurobiology Imaging and Tissue Processing Core (Core C) provides state-of-the-artequipment and technical support for experimental projects on the assembly and modulation of synaptic,neuronal, and glial structure and function. By sharing technical expertise, equipment, facilities, andprofessional staff, the Core facilitates cost-effective, cross-project collaborations. The DevelopmentalNeurobiology Imaging and Tissue Processing Core performs cytological and histological processing ofexperimental tissues, and performs image analysis in a regular and reliable fashion for the developmentalsynaptic neurobiology, neuroimmunoendocrinology and infectious disease, and molecular biological andgenetic studies projects outlined in the MRRC. In particular, the Developmental Neurobiology Imaging andTissue Processing Core provides a facility for cell and tissue processing and quantitative image analysis atthe light, confocal, and electron microscopic levels for many projects comprising the MRRC portfolio. In thisregard, the Imaging Core contributes to an interrelationship and synergism among the component projects,resulting in greater scientific productivity and improved cost effectiveness than individual projects couldachieve separately. The currently re-configured Neurobiology Tissue Processing and Imaging Core is theresult of a merger of two previously existing cores (developmental neurobiology and tissue processing ANDcombined LSCM imaging and electrophysiology) that had two clearly delineated objectives from the originalP30. For many investigators, these two objectives are sequential and therefore have led to a sequentialuse of two non-overlapping facilities. Now, these tasks are integrated allowing an investigator toaccomplish cell or tissue processing and various imaging modalities including: time-lapse live imaging forstable, long-term high-resolution morphological studies; simultaneous real time two photon laser scanningconfocal microscopy imaging and recording of plasma membrane potential by the whole-cell patch-clamptechnique; monitoring of presynaptic release probability of individual synapses by the de-staining rate of theFM fluorescent dyes; high resolution electron microscopy including serial section analysis of reconstructedcells; and combined immunohistochemical staining and electron microscopy for subcellular localization ofepitopes a single unit. These techniques require constantly evolving skills on the side of the investigatorsand a significant investment in instrumentation.
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