This proposal seeks to continue and expand technological biomedical research, collaborative, service, training and dissemination activities ofthe Laboratory for Fluorescence Dynamics (LFD). Since 1986, the LFD has been a national resource center dedicated to 1) develop of new fluorescence technologies for biology and medicine and 2) service, training, and dissemination of fluorescence methods in a user facility. In previous years (1986-2010), we established our reputation as the leaders in technological development for fluorescence dynamics. Since moving to UCI in 2006, we expanded the core research and collaborative work in this new fertile biomedical research environment. We created stmctures for training through workshops, tutorials and organization of LFD training courses at UCI and other US universities. Core development expands current limits of biomedically relevant fluorescence instrumentation, particularly for the study of cellular processes. Projects include: 1) new concepts in fluorescence microscopy that develop novel imaging methods to exploit the dynamic fluorescence methodologies that are the hallmark ofthe LFD developments, 2) expanding the concept of spatio-temporal fluctuation correlation analysis to a new powerful image approach that provides detailed information of molecular flow at the nanoscale, 3) new methods for 3D nanoimaging based on the orbital tracking approach, 4) novel technologies for fluorescence lifetime imaging analysis that allow new contrast mechanisms to be applied to tissues and animals, and 5) development of software/algorithm platform for disseminating the methods for data collection and analysis developed at the LFD. Users atthe LFD find ready access to new and innovative technology, which affords them a unique opportunity to rapidly advance their own research programs. Driving biological problems exploit and push technological advances and research in biological processes, macromolecular assembly, tissue organization and dynamics, membrane morphology/function relationships, and innovative biomedical instrumentation. Educational programs include hands-on training of students, postdoctoral fellows and visiting scientists, workshops and specialized schools in innovative fluorescence methodologies.

Public Health Relevance

The proposed technological developments provide real time detection and localization of molecular interactions in 3D in live cells and tissues. We have obtained technological breakthroughs in the areas of microscopy imaging, algorithm developments for revealing comprehensive spatio-temporal correlations, nanoimaging methods with unprecedented resolution and new spectroscopic contrast methods that can identify metabolic states of cells and predict their behavior.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Biotechnology Resource Grants (P41)
Project #
5P41GM103540-28
Application #
8499383
Study Section
Special Emphasis Panel (ZRG1-BCMB-K (40))
Program Officer
Friedman, Fred K
Project Start
1997-08-01
Project End
2016-06-30
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
28
Fiscal Year
2013
Total Cost
$1,124,120
Indirect Cost
$407,458
Name
University of California Irvine
Department
Biomedical Engineering
Type
Schools of Engineering
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697
Sosnik, Julian; Zheng, Likun; Rackauckas, Christopher V et al. (2016) Noise modulation in retinoic acid signaling sharpens segmental boundaries of gene expression in the embryonic zebrafish hindbrain. Elife 5:e14034
Lin, Sophia; Hedde, Per Niklas; Venugopalan, Vasan et al. (2016) Multi-scale silica structures for improved HIV-1 Capsid (p24) antigen detection. Analyst 141:4181-8
Wang, Bo; Rong, Xin; Duerr, Mark A et al. (2016) Intestinal Phospholipid Remodeling Is Required for Dietary-Lipid Uptake and Survival on a High-Fat Diet. Cell Metab 23:492-504
Sameni, Sara; Syed, Adeela; Marsh, J Lawrence et al. (2016) The phasor-FLIM fingerprints reveal shifts from OXPHOS to enhanced glycolysis in Huntington Disease. Sci Rep 6:34755
Datta, Rupsa; Heylman, Christopher; George, Steven C et al. (2016) Label-free imaging of metabolism and oxidative stress in human induced pluripotent stem cell-derived cardiomyocytes. Biomed Opt Express 7:1690-701
Hinde, Elizabeth; Pandžić, Elvis; Yang, Zhengmin et al. (2016) Quantifying the dynamics of the oligomeric transcription factor STAT3 by pair correlation of molecular brightness. Nat Commun 7:11047
Szymanska, Agnieszka F; Heylman, Christopher; Datta, Rupsa et al. (2016) Automated detection and analysis of depolarization events in human cardiomyocytes using MaDEC. Comput Biol Med 75:109-17
Alfonso-García, Alba; Smith, Tim D; Datta, Rupsa et al. (2016) Label-free identification of macrophage phenotype by fluorescence lifetime imaging microscopy. J Biomed Opt 21:46005
Moore, J S; Xantheas, S S; Grate, J W et al. (2016) Modular Polymer Biosensors by Solvent Immersion Imprint Lithography. J Polym Sci B Polym Phys 54:98-103
Clark, Natalie M; Hinde, Elizabeth; Winter, Cara M et al. (2016) Tracking transcription factor mobility and interaction in Arabidopsis roots with fluorescence correlation spectroscopy. Elife 5:

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