This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Smoking induced endobronchial and oral cancers are leading causes of tobacco induced mortality. More accurate methods for diagnosis are needed to improve treatment and survival. We are developing-high resolution (near histological level) flexible fiberoptic endoscopic optical coherence tomographic methods for oral and airway cancer detection and monitoring. Optical coherence tomography (OCT) and optical Doppler tomography use interferometric broad-band coherent gating reflectance techniques to obtain high resolution structural and capillary flow dynamics at defined high resolution spatial locations even in highly scattering tissues like oral mucosa and airways. The 2-10 um OCT resolution capabilities enable us to begin investigation of non-invasive optical imaging of in-vivo tissue structure and microcirculation for diagnosis of oral and airway cancers. We propose to improve diagnosis and treatment of smoking induced airway and oral cancers by further development of flexible fiberoptic OCT/ODT for pulmonary and oral oncologic applications.
Specific Aims : 1. To develop real-time flexible fiberoptic bronchoscopic methods for high-resolution simultaneous OCT/ODT evaluation of airway and oral tumors. 2. Evaluate these OCT/ODT capabilities for identifying oral cancers in live animal models and airway cancers in patients with smoking induced airway malignancy. 3. Assess the potential for combining low resolution in-vivo tumor fluorescence localization with OCT/ODT high resolution imaging to improve diagnostic yield and detection efficiency for oral and airway cancers, and to better understand functional optical markers for malignant transformation. 4. Develop methods for ultra-high resolution Ti:sapphire in-vivo OCT imaging.
|Malacrida, Leonel; Astrada, Soledad; Briva, Arturo et al. (2016) Spectral phasor analysis of LAURDAN fluorescence in live A549 lung cells to study the hydration and time evolution of intracellular lamellar body-like structures. Biochim Biophys Acta 1858:2625-2635|
|Choi, Bernard; Tan, Wenbin; Jia, Wangcun et al. (2016) The Role of Laser Speckle Imaging in Port-Wine Stain Research: Recent Advances and Opportunities. IEEE J Sel Top Quantum Electron 2016:|
|Alfonso-GarcÃa, Alba; Pfisterer, Simon G; Riezman, Howard et al. (2016) D38-cholesterol as a Raman active probe for imaging intracellular cholesterol storage. J Biomed Opt 21:61003|
|Alfonso-GarcÃa, Alba; Smith, Tim D; Datta, Rupsa et al. (2016) Label-free identification of macrophage phenotype by fluorescence lifetime imaging microscopy. J Biomed Opt 21:46005|
|Libby, Andrew E; Wang, Hong; Mittal, Richa et al. (2015) Lipoprotein lipase is an important modulator of lipid uptake and storage in hypothalamic neurons. Biochem Biophys Res Commun 465:287-92|
|Datta, Rupsa; Alfonso-GarcÃa, Alba; Cinco, Rachel et al. (2015) Fluorescence lifetime imaging of endogenous biomarker of oxidative stress. Sci Rep 5:9848|
|Badran, Karam W; Manuel, Cyrus T; Loy, Anthony Chin et al. (2015) Long-term in vivo electromechanical reshaping for auricular reconstruction in the New Zealand white rabbit model. Laryngoscope 125:2058-66|
|Chlebicki, Cara A; Protsenko, Dmitry E; Wong, Brian J (2014) Preliminary investigations on therapy thresholds for laser dosimetry, cryogen spray cooling duration, and treatment cycles for laser cartilage reshaping in the New Zealand white rabbit auricle. Lasers Med Sci 29:1099-109|
|Rohde, S B; Kim, A D (2014) Convolution model of the diffuse reflectance for layered tissues. Opt Lett 39:154-7|
|Rohrbach, Daniel J; Muffoletto, Daniel; Huihui, Jonathan et al. (2014) Preoperative mapping of nonmelanoma skin cancer using spatial frequency domain and ultrasound imaging. Acad Radiol 21:263-70|
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