Research conducted over the past 50 years has provided considerable knowledge regarding the chemical constituents of smokeless tobacco products, smoked tobacco products and cigarette smoke. Meanwhile, there is a paucity of data regarding the microbial constituents of tobacco and smoke, and their associated adverse health effects. Yet, tobacco microbial constituents may play significant roles in the development of both infectious and chronic diseases among tobacco users. To address these knowledge gaps, the longterm objective of this proposed project is to harness the power of next-generation sequencing technologies to increase our understanding of the microbial constituents of conventional, new and emerging tobacco products, and greatly improve our understanding of microbial-related health risks among tobacco users.
The specific aims of the proposal are as follows: 1) To explore the bacterial microbiome of conventional, new and manipulated smoked and smokeless tobacco products and smoke, and examine the role of specific genera in tobacco-specific N-nitrosamine formation;2) To provide novel, baseline data on the composition of the oral microbiome and its associated expressed activities in smokers and smokeless tobacco users compared with that of non-users;and 3) To characterize the transient changes-bacterial community composition and expressed metabolic activities-in the oral microbiome after single-use of new and manipulated smoked and smokeless tobacco products. To accomplish these aims, this study will employ an innovative, polyphasic approach involving the application of next-generation sequencing methods in: 1) time course experiments with conventional, new and manipulated tobacco products;2) a longitudinal study of tobacco users and nonusers;and 3) cross-over trials of tobacco users testing new or manipulated tobacco products. This approach will generate a rich, novel and valuable data source that can be immediately utilized by FDA as it implements the Family Smoking Prevention and Tobacco Control Act and seeks to improve tobacco regulation for the benefit of public health. Specifically, these data could inform potential new microbial-related tobacco regulations that have never before been considered by U.S. governmental regulators.
The data generated in this innovative project will improve scientific knowledge of the role that tobacco bacterial communities may play in the development of both acute infectious diseases and chronic diseases among tobacco users. This knowledge will fill a critical gap in the field of tobacco regulatory science which has yet to address the possible public health implications associated with microbial constituents of tobacco.
|Ghosh, Arijit; Woolum, Karen; Knopp, Michael V et al. (2018) Development and optimization of a novel automated loop method for production of [11C]nicotine. Appl Radiat Isot 140:76-82|
|Halpern-Felsher, Bonnie; Kim, Hyoshin (2018) Measuring E-cigarette use, dependence, and perceptions: Important principles and considerations to advance tobacco regulatory science. Addict Behav 79:201-202|
|Mead, Erin L; Cruz-Cano, Raul; Groom, Allison et al. (2018) Responses to cigarette health warning labels, harm perceptions and knowledge in a national sample of pregnant and non-pregnant women of reproductive age. Addict Behav 90:10-13|
|Mead, Erin L; Johnson, Sarah Lindstrom; Siddiqui, Junaed et al. (2018) Beyond blunts: Reasons for cigarette and cigar use among African American young adult dual users. Addict Res Theory 26:349-360|
|Mikheev, Vladimir B; Buehler, Stephanie S; Brinkman, Marielle C et al. (2018) The application of commercially available mobile cigarette topography devices for e-cigarette vaping behavior measurements. Nicotine Tob Res :|
|Weaver, Scott R; Kim, Hyoshin; Glasser, Allison M et al. (2018) Establishing consensus on survey measures for electronic nicotine and non-nicotine delivery system use: Current challenges and considerations for researchers. Addict Behav 79:203-212|
|Hamad, Samera H; Johnson, Nathan M; Tefft, Margaret E et al. (2017) Little Cigars vs 3R4F Cigarette: Physical Properties and HPHC Yields. Tob Regul Sci 3:459-478|
|Chopyk, Jessica; Chattopadhyay, Suhana; Kulkarni, Prachi et al. (2017) Mentholation affects the cigarette microbiota by selecting for bacteria resistant to harsh environmental conditions and selecting against potential bacterial pathogens. Microbiome 5:22|
|Kim, Hyoshin; Davis, Andrew H; Dohack, Jaime L et al. (2017) E-Cigarettes Use Behavior and Experience of Adults: Qualitative Research Findings to Inform E-Cigarette Use Measure Development. Nicotine Tob Res 19:190-196|
|Chen, Julia Cen; Das, Babita; Mead, Erin L et al. (2017) Flavored E-cigarette Use and Cigarette Smoking Susceptibility among Youth. Tob Regul Sci 3:68-80|
Showing the most recent 10 out of 20 publications