CORE ABSTRACT The purpose of the Bioanalytical Core is to provide analytical capability for Projects #1-4 in human subjects and murine models of sepsis, including genomic, biochemical, and cell-functional analysis of isolated leukocyte populations, plasma, and tissue(s) of interest (e.g., skeletal muscle). The Core will: 1) characterize leukocyte populations phenotypically;2) measure acute-phase protein, plasma cytokine, and select growth factor concentrations;3) obtain a consistent genome-wide and gene-specific expression analysis profile from enriched cell populations and tissues;4) assess protein catabolism and mitochondrial bioenergetics in muscle tissues by measuring mitochondrial function, reactive oxygen species, and catabolism-atrophy related pathways;and 5) provide a central scientific analysis platform for all projects.
Aim 1. To phenotype blood and tissue leukocytes from humans and murine models of sepsis. We will focus on early myeloid cell populations derived from whole blood in humans with sepsis, and from bone marrow and spleen in mice with sepsis.
Aim 2. To provide plasma or serum acute-phase protein, cytokine, and growth factor concentration measurements for clinical and laboratory studies using Luminex xMAP" technology and ELISA. We will use a MILLIPLEX Analyzer 3.1 xPONENT" System and standard ELISA microplate readers.
Aim 3. To determine genome-wide and individual gene expression in both human and murine leukocytes and select tissue samples. We will assess exon-level, genome-wide expression with Affymetrix GeneChip(R) technology (HH/2) and determine expression of selected leukocyte genes by nanoString nCounter(R).
Aim 4. To assess catabolism, bioenergetics, and select pathways of atrophy with novel and standard methodologies. The methodologies will allow us to better understand molecular and biochemical changes related to muscle atrophy and energy decline in human subjects and murine models.
Aim 5. To develop innovative new methodologies and provide a central scientific platform for future discovery. The Core will collaborate with all projects for the integration of dynamic scientific knowledge and discovery and decide on newly innovative methodologies for future analysis. Additionally, the Analytical Studies Core will centralize the analytical capabilities that require sophistication and quality assurance beyond that available to individual projects. It will assure accuracy and standardization of complex analytical protocols that will be cost-efficient for the P50 Center.
|Mathias, Brittany; Delmas, Amber L; Ozrazgat-Baslanti, Tezcan et al. (2016) Human Myeloid-derived Suppressor Cells are Associated With Chronic Immune Suppression After Severe Sepsis/Septic Shock. Ann Surg :|
|Loftus, Tyler J; Brakenridge, Scott C; Moore, Frederick A et al. (2016) Routine surveillance cholangiography following percutaneous cholecystostomy delays drain removal and cholecystectomy. J Trauma Acute Care Surg :|
|Loftus, Tyler J; Jordan, Janeen R; Croft, Chasen A et al. (2016) Temporary abdominal closure for trauma and intra-abdominal sepsis: different patients, different outcomes. J Trauma Acute Care Surg :|
|Mankowski, Robert T; Ahmed, Shakeel; Beaver, Thomas et al. (2016) Intraoperative hemidiaphragm electrical stimulation reduces oxidative stress and upregulates autophagy in surgery patients undergoing mechanical ventilation: exploratory study. J Transl Med 14:305|
|Lopez, Maria-Cecilia; Efron, Philip A; Ozrazgat-Baslanti, Tezcan et al. (2016) Sex-based differences in the genomic response, innate immunity, organ dysfunction, and clinical outcomes after severe blunt traumatic injury and hemorrhagic shock. J Trauma Acute Care Surg 81:478-85|
|Korenkevych, Dmytro; Ozrazgat-Baslanti, Tezcan; Thottakkara, Paul et al. (2016) The Pattern of Longitudinal Change in Serum Creatinine and 90-Day Mortality After Major Surgery. Ann Surg 263:1219-27|
|Loftus, Tyler J; Go, Kristina L; Hughes, Steven J et al. (2016) Improved outcomes following implementation of an acute gastrointestinal bleeding multidisciplinary protocol. J Trauma Acute Care Surg :|
|Mira, Juan C; Szpila, Benjamin E; Nacionales, Dina C et al. (2016) Patterns of gene expression among murine models of hemorrhagic shock/trauma and sepsis. Physiol Genomics 48:135-44|
|Alamo, Ines G; Kannan, Kolenkode B; Ramos, Harry et al. (2016) Clonidine reduces norepinephrine and improves bone marrow function in a rodent model of lung contusion, hemorrhagic shock, and chronic stress. Surgery :|
|Mathias, Brittany; Lipori, Gigi; Moldawer, Lyle L et al. (2016) Integrating "big data" into surgical practice. Surgery 159:371-4|
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