The CARC's Molecular/Cellular Biology Laboratory Core performs a fundamental role in coordinating the research activities of CARC research components and pilot projects. In addition, the Core will extend its expertise to support NIAAA funded projects that tie to the theme of the CARC. Its mission will be to serve as a core resource for conducting experimental procedures, training technical personnel, and developing cutting-edge analytical techniques that enable our investigators to achieve their CARC research objectives.
The Specific Aims of the Molecular/Cellular Biology Laboratory Core are: 1. To perform cellular phenotyping, functional analysis, and sorting using fluorescent activated cell-sorting (FACS) facilities. 2. To perform molecular biological assays and procedures as needed by the CARC investigators. 3. To perform specific analytical assays for projects supported by the CARC. 4. To maintain a cell culture facility and support research activities utilizing cell culture technology. In addition to the four Specific Aims, the CARC Molecular/Cellular Biology Laboratory Core has an established track record of expanding the repertoire of flow cytometry, gene cloning, Taqman assays, luminex assays, and ELISAs available to CARC investigators. Developmental aspects of the Core will be focused on CARC objectives in order to ensure their succress. These include 1) developing novel Taqman PCR and RT-PCR assays linked to FACS sorting of rare cell events and 2) collaborating with the Geneomics Core Facility at LSUHSC to expand analytical protocols for determination of DNA methylation and chromatin acetylation in the regulation of specific gene expression. Priorities will be enforced to secure maximal productivity of research projects supported by the Center. The personnel in this core will train other personnel and will help coordinate research efforts among projects. The overall goal of the Molecular/Cellular Biology Laboratory Core is to enhance efficiency, quality control, cooperation, and flexibility of research participants in their pursuit of Center objectives.

Public Health Relevance

The Molecular/Cellular Biology Laboratory Core performs a fundamental role in coordinating the investigations of the CARC. Novel information obtained from these studies will greatly advance our knowledge about the adverse effects of alcohol abuse on the development of HIV/AIDS and anti-retroviral therapy treatment of this infection in alcohol abusers

Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Comprehensive Center (P60)
Project #
5P60AA009803-21
Application #
8577111
Study Section
Special Emphasis Panel (ZAA1-DD)
Project Start
Project End
Budget Start
2013-12-01
Budget End
2014-11-30
Support Year
21
Fiscal Year
2014
Total Cost
$212,823
Indirect Cost
$58,014
Name
Louisiana State Univ Hsc New Orleans
Department
Type
DUNS #
782627814
City
New Orleans
State
LA
Country
United States
Zip Code
70112
Kaplan, Kathryn C; Hormes, Julia M; Wallace, Maeve et al. (2016) Racial Discrimination and HIV-related Risk Behaviors in Southeast Louisiana. Am J Health Behav 40:132-43
Molina, Patricia E (2016) Mechanisms Involved in Disruption of Adipose Tissue Mass Resulting from Chronic Unhealthy Alcohol Consumption. Alcohol Clin Exp Res 40:2296-2298
Guizzetti, Marina; Davies, Daryl L; Egli, Mark et al. (2016) Sex and the Lab: An Alcohol-Focused Commentary on the NIH Initiative to Balance Sex in Cell and Animal Studies. Alcohol Clin Exp Res 40:1182-91
de la Rua, Nicholas M; Samuelson, Derrick R; Charles, Tysheena P et al. (2016) CD4(+) T-Cell-Independent Secondary Immune Responses to Pneumocystis Pneumonia. Front Immunol 7:178
Bruce-Keller, Annadora J; Salbaum, J Michael; Luo, Meng et al. (2016) Reply to: High-Fat Diet-Induced Dysbiosis as a Cause of Neuroinflammation. Biol Psychiatry 80:e5-6
Jolley, Sarah E; Alkhafaf, Qasim; Hough, Catherine et al. (2016) Presence of an Alcohol Use Disorder is Associated with Greater Pneumonia Severity in Hospitalized HIV-Infected Patients. Lung 194:755-62
Simon, Liz; Song, Keijing; Vande Stouwe, Curtis et al. (2016) Δ9-Tetrahydrocannabinol (Δ9-THC) Promotes Neuroimmune-Modulatory MicroRNA Profile in Striatum of Simian Immunodeficiency Virus (SIV)-Infected Macaques. J Neuroimmune Pharmacol 11:192-213
Fleckman, Julia M; Drury, Stacy S; Taylor, Catherine A et al. (2016) Role of Direct and Indirect Violence Exposure on Externalizing Behavior in Children. J Urban Health 93:479-92
Goldsmith, Felicia; Guice, Justin; Page, Ryan et al. (2016) Obese ZDF rats fermented resistant starch with effects on gut microbiota but no reduction in abdominal fat. Mol Nutr Food Res :
Robichaux, Spencer; Lacour, Nedra; Bagby, Gregory J et al. (2016) Validation of RPS13 as a reference gene for absolute quantification of SIV RNA in tissue of rhesus macaques. J Virol Methods 236:245-51

Showing the most recent 10 out of 84 publications