The CARC's Molecular/Cellular Biology Laboratory Core performs a fundamental role in coordinating the research activities of CARC research components and pilot projects. In addition, the Core will extend its expertise to support NIAAA funded projects that tie to the theme of the CARC. Its mission will be to serve as a core resource for conducting experimental procedures, training technical personnel, and developing cutting-edge analytical techniques that enable our investigators to achieve their CARC research objectives.
The Specific Aims of the Molecular/Cellular Biology Laboratory Core are: 1. To perform cellular phenotyping, functional analysis, and sorting using fluorescent activated cell-sorting (FACS) facilities. 2. To perform molecular biological assays and procedures as needed by the CARC investigators. 3. To perform specific analytical assays for projects supported by the CARC. 4. To maintain a cell culture facility and support research activities utilizing cell culture technology. In addition to the four Specific Aims, the CARC Molecular/Cellular Biology Laboratory Core has an established track record of expanding the repertoire of flow cytometry, gene cloning, Taqman assays, luminex assays, and ELISAs available to CARC investigators. Developmental aspects of the Core will be focused on CARC objectives in order to ensure their succress. These include 1) developing novel Taqman PCR and RT-PCR assays linked to FACS sorting of rare cell events and 2) collaborating with the Geneomics Core Facility at LSUHSC to expand analytical protocols for determination of DNA methylation and chromatin acetylation in the regulation of specific gene expression. Priorities will be enforced to secure maximal productivity of research projects supported by the Center. The personnel in this core will train other personnel and will help coordinate research efforts among projects. The overall goal of the Molecular/Cellular Biology Laboratory Core is to enhance efficiency, quality control, cooperation, and flexibility of research participants in their pursuit of Center objectives.
The Molecular/Cellular Biology Laboratory Core performs a fundamental role in coordinating the investigations of the CARC. Novel information obtained from these studies will greatly advance our knowledge about the adverse effects of alcohol abuse on the development of HIV/AIDS and anti-retroviral therapy treatment of this infection in alcohol abusers
|Choi, Sun-Mi; McAleer, Jeremy P; Trevejo-Nunez, Giraldina et al. (2014) Acute alcohol intoxication impairs methicillin-resistant Staphylococcus aureus clearance in the lung by impeding epithelial production of Reg3?. Infect Immun 82:1402-7|
|Siggins, Robert W; Molina, Patricia; Zhang, Ping et al. (2014) Dysregulation of myelopoiesis by chronic alcohol administration during early SIV infection of rhesus macaques. Alcohol Clin Exp Res 38:1993-2000|
|Brown, Armand O; Mann, Beth; Gao, Geli et al. (2014) Streptococcus pneumoniae translocates into the myocardium and forms unique microlesions that disrupt cardiac function. PLoS Pathog 10:e1004383|
|Dodd, Tracy; Simon, Liz; LeCapitaine, Nicole J et al. (2014) Chronic binge alcohol administration accentuates expression of pro-fibrotic and inflammatory genes in the skeletal muscle of simian immunodeficiency virus-infected macaques. Alcohol Clin Exp Res 38:2697-706|
|Loganantharaj, Nisha; Nichols, Whitney A; Bagby, Gregory J et al. (2014) The effects of chronic binge alcohol on the genital microenvironment of simian immunodeficiency virus-infected female rhesus macaques. AIDS Res Hum Retroviruses 30:783-91|
|Ghare, Smita S; Joshi-Barve, Swati; Moghe, Akshata et al. (2014) Coordinated histone H3 methylation and acetylation regulate physiologic and pathologic fas ligand gene expression in human CD4+ T cells. J Immunol 193:412-21|
|Molina, Patricia E (2014) Alcohol binging exacerbates adipose tissue inflammation following burn injury. Alcohol Clin Exp Res 38:33-5|
|Deshmukh, Hitesh S; Liu, Yuhong; Menkiti, Ogechukwu R et al. (2014) The microbiota regulates neutrophil homeostasis and host resistance to Escherichia coli K1 sepsis in neonatal mice. Nat Med 20:524-30|
|Simon, Liz; LeCapitaine, Nicole; Berner, Paul et al. (2014) Chronic binge alcohol consumption alters myogenic gene expression and reduces in vitro myogenic differentiation potential of myoblasts from rhesus macaques. Am J Physiol Regul Integr Comp Physiol 306:R837-44|
|El Hajj, Elia C; El Hajj, Milad C; Voloshenyuk, Tetyana G et al. (2014) Alcohol modulation of cardiac matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs favors collagen accumulation. Alcohol Clin Exp Res 38:448-56|
Showing the most recent 10 out of 37 publications