Human cytomegalovirus (HCMV) is one of the most common opportunistic infectious agents in AIDS patients. Viral infections in the spleen are believed to contribute to pathogenesis and virulence of systemic infections since highly active viral replication in the spleen, common in immunocompromised hosts, usually leads to lesions in the organ and impairs its function for control of viral infection. During primary infection, the viruses produced in the spleen disseminate to other organs and represent one of the major sources for subsequent infection of many organs. Moreover, the spleen is also a site for viral persistent and latent infections. Thus, CMV replication in the spleen is a major determinant of viral virulence and pathogenesis. However, little is currently known about the mechanism of how CMV replicates in the spleen. Equally elusive is the nature of viral determinants required for CMV infection in the organ. Using murine cytomegalovirus (MCMV) infection in immunodeficient animals as a model system, the proposed research is to study viral genes required for CMV replication in spleen and to investigate the roles of these viral determinants in supporting CMV infections in splenic tissues and cells. We have recently developed a novel approach to construct MCMV mutants and have isolated two novel mutants that are defective in growth in spleens and exhibit little virulence in killing immunodeficient animals. One mutant was attenuated to grow in splenic cells while the other failed to modulate NK cell-mediated response in spleens. In the proposed research, we will first study how these two mutants are defective in infecting spleen tissues and cells. We will then characterize the mutated ORFs, which encode MCMV determinants for viral infection in spleen. Furthermore, host factors that potentially interact with these viral ORFs will be identified and their interactions with viral proteins will be examined. Finally, we will investigate the mechanism of how the identified viral determinants function in supporting MCMV infections in spleens, including in modulating dendritic cells and in blocking NKG2D-ligand interactions. These studies will characterize viral determinants for infection in spleens and elucidate the role of these genes in enhancing viral virulence and causing CMV systemic infection. Studying viral virulence factors and understanding their roles in CMV pathogenesis will provide insight into developing novel approaches for the treatment of CMV-associated complications, including those associated with AIDS patients.

Public Health Relevance

Human cytomegalovirus (CMV) causes one of the most common opportunistic infections encountered in AIDS patients, and viral infection in spleen is primarily responsible for CMV virulence in immunodeficient hosts. The objective of the proposed studies is to identify the cytomegalovirus (CMV) genes that are important for viral infection in the spleen, and to study the mechanism of viral replication in the spleen tissue. Our study will facilitate the development of new drugs and novel approaches for treating CMV infection by blocking the expression and function of these identified genes.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Project (R01)
Project #
Application #
Study Section
AIDS-associated Opportunistic Infections and Cancer Study Section (AOIC)
Program Officer
Lambros, Chris
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of California Berkeley
Internal Medicine/Medicine
Schools of Public Health
United States
Zip Code
Feng, Linyuan; Sheng, Jingxue; Vu, Gia-Phong et al. (2018) Human cytomegalovirus UL23 inhibits transcription of interferon-? stimulated genes and blocks antiviral interferon-? responses by interacting with human N-myc interactor protein. PLoS Pathog 14:e1006867
Zhu, Dihan; Pan, Chaoyun; Sheng, Jingxue et al. (2018) Human cytomegalovirus reprogrammes haematopoietic progenitor cells into immunosuppressive monocytes to achieve latency. Nat Microbiol 3:503-513
Liu, Guoyu; Hai, Rong; Liu, Fenyong (2017) Detection of congenital cytomegalovirus in newborns using nucleic acid amplification techniques and its public health implications. Virol Sin 32:376-386
Lei, Lei; Wang, Wenbiao; Xia, Chuan et al. (2016) Salmonella Virulence Factor SsrAB Regulated Factor Modulates Inflammatory Responses by Enhancing the Activation of NF-?B Signaling Pathway. J Immunol 196:792-802
Zhang, Tianfu; Yu, Jianxiong; Zhang, Yaqin et al. (2014) Salmonella enterica serovar enteritidis modulates intestinal epithelial miR-128 levels to decrease macrophage recruitment via macrophage colony-stimulating factor. J Infect Dis 209:2000-11
Yang, Zhu; Mao, Guoliang; Liu, Yujun et al. (2013) Detection of the pandemic H1N1/2009 influenza A virus by a highly sensitive quantitative real-time reverse-transcription polymerase chain reaction assay. Virol Sin 28:24-35
Luo, Jun; Chen, Jun; Yang, Edward et al. (2013) Modulation of the cellular distribution of human cytomegalovirus helicase by cellular factor Snapin. J Virol 87:10628-40
Pei, Yonggang; Fu, Wenmin; Yang, Ed et al. (2012) A Hsp40 chaperone protein interacts with and modulates the cellular distribution of the primase protein of human cytomegalovirus. PLoS Pathog 8:e1002968
Jiang, Xiaohong; Chen, Yuan-Chuan; Gong, Hao et al. (2012) Ribonuclease P-mediated inhibition of human cytomegalovirus gene expression and replication induced by engineered external guide sequences. RNA Biol 9:1186-95
Shen, Ao; Lei, Ji; Yang, Edward et al. (2011) Human cytomegalovirus primase UL70 specifically interacts with cellular factor Snapin. J Virol 85:11732-41

Showing the most recent 10 out of 15 publications