Methionine sulfoxide reductases (Msr's) catalyze the reduction of methionine sulfoxide back to (normal) methionine. The result is reactivation of oxidatively damaged proteins and this activity is known to facilitate successful gastric colonization by H. pylori. This project is designed to identify the critical amino acid residues associated with three identified H. pylori proteins that undergo this reductive repair process. Two types of functional residues will be identified. These are the specific Met residues repaired by Msr, and the residues involved in recognizing Msr. The key types of residues will be identified by studying pure H. pylori Msr and its interaction with identified target proteins. The information from the pure protein studies will be used to assess the degree of oxidative Met residue damage in the target proteins upon stress agent exposure of whole cells. Then the information on the individual target proteins docking sites (Msr-interacting sites) will be used to understand the need for each target proteins repair in H. pylori stress physiology and stomach colonizing abilities.

Public Health Relevance

Methionine repair by the persistent human pathogen Helicobacter pylori is important for the bacterium to survive in the stomach. The mechanisms used to repair methionine-rich proteins and the physiological consequences of such repair will be uncovered so that measures to counteract the pathogen can be developed.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI077569-04
Application #
8197120
Study Section
Bacterial Pathogenesis Study Section (BACP)
Program Officer
Mills, Melody
Project Start
2008-12-01
Project End
2013-11-30
Budget Start
2011-12-01
Budget End
2012-11-30
Support Year
4
Fiscal Year
2012
Total Cost
$361,412
Indirect Cost
$116,387
Name
University of Georgia
Department
Microbiology/Immun/Virology
Type
Schools of Arts and Sciences
DUNS #
004315578
City
Athens
State
GA
Country
United States
Zip Code
30602
Benoit, St├ęphane L; Maier, Robert J (2014) Twin-arginine translocation system in Helicobacter pylori: TatC, but not TatB, is essential for viability. MBio 5:e01016-13
Kuhns, Lisa G; Mahawar, Manish; Sharp, Joshua S et al. (2013) Role of Helicobacter pylori methionine sulfoxide reductase in urease maturation. Biochem J 450:141-8
Benoit, St├ęphane L; Bayyareddy, Krishnareddy; Mahawar, Manish et al. (2013) Alkyl hydroperoxide reductase repair by Helicobacter pylori methionine sulfoxide reductase. J Bacteriol 195:5396-401
Mahawar, Manish; Tran, ViLinh; Sharp, Joshua S et al. (2011) Synergistic roles of Helicobacter pylori methionine sulfoxide reductase and GroEL in repairing oxidant-damaged catalase. J Biol Chem 286:19159-69
Wang, Ge; Lo, Leja F; Maier, Robert J (2011) The RecRO pathway of DNA recombinational repair in Helicobacter pylori and its role in bacterial survival in the host. DNA Repair (Amst) 10:373-9
Wang, Ge; Maier, Susan E; Lo, Leja F et al. (2010) Peptidoglycan deacetylation in Helicobacter pylori contributes to bacterial survival by mitigating host immune responses. Infect Immun 78:4660-6