New Targets for Reproductive Control of Mosquito Vectors. In this project, we will investigate the male seminal fluid proteins (SFPs) of two major disease vectors of dengue and Chikungunya viruses: the yellow fever mosquito (Aedes aegypti) and the Asian tiger mosquito (Aedes albopictus). Both dengue and Chikungunya are increasing in incidence globally and there is an immediate need to develop novel and effective vector control strategies for these two species. In this study we will conduct two specific aims to identify and functionally characterize SFPs, for the first time, in Ae. aegypti and Ae. albopictus. Our goal is to directly identify new mosquito SFP targets that could be manipulated as a novel means of reproductive control, or to modify the blood feeding physiology of insects that transmit deadly pathogens, with the long-term goal to reduce or eliminate virus transmission to people.

Public Health Relevance

In this study, we will directly identify and determine the function of seminal fluid proteins produced in male mosquitoes and transferred to females during mating. We will identify targets that could be manipulated as a novel means of reproductive control or as a means of modifying the blood feeding physiology of insects that transmit deadly pathogens causing diseases such as dengue and Chikungunya, with the long-term goal to reduce or eliminate virus transmission to people.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI095491-02
Application #
8265607
Study Section
Vector Biology Study Section (VB)
Program Officer
Costero, Adriana
Project Start
2011-06-01
Project End
2016-05-31
Budget Start
2012-06-01
Budget End
2013-05-31
Support Year
2
Fiscal Year
2012
Total Cost
$419,531
Indirect Cost
$116,176
Name
Cornell University
Department
Zoology
Type
Schools of Earth Sciences/Natur
DUNS #
872612445
City
Ithaca
State
NY
Country
United States
Zip Code
14850
Alfonso-Parra, Catalina; Avila, Frank W; Deewatthanawong, Prasit et al. (2014) Synthesis, depletion and cell-type expression of a protein from the male accessory glands of the dengue vector mosquito Aedes aegypti. J Insect Physiol 70:117-24
Perry, Jennifer C; Sirot, Laura; Wigby, Stuart (2013) The seminal symphony: how to compose an ejaculate. Trends Ecol Evol 28:414-22
Helinski, Michelle E H; Valerio, Laura; Facchinelli, Luca et al. (2012) Evidence of polyandry for Aedes aegypti in semifield enclosures. Am J Trop Med Hyg 86:635-41