The long-term objective of this project is to determine the function(s) of dystrophin in striated muscle in order to understand how its absence or abnormality leads to the pathologies observed in Duchenne (DMD) and Becker (BMD) muscular dystrophies. During the most recent project period, we uniquely employed the baculovirus expression system and an array of biochemical/biophysical assays to define the actin binding properties of dystrophin and its homologue utrophin. We also demonstrated that dystrophin functions to organize the microtubule lattice of skeletal muscle through a direct binding interaction. Finally, we demonstrated that a baculovirus-expressed TAT-micro-utrophin construct shows promise as a novel protein replacement therapy for DMD. Based on these published results and exciting new preliminary data, we propose three new specific aims to: 1) test the hypothesis that disease-causing missense mutations in dystrophin cause a loss of function through protein aggregation, 2) determine the functional importance of microtubule lattice organization in skeletal muscle, and 3) reinvestigate functional necessity for the dystrophin carboxyl-terminal domain in skeletal muscle. The project will employ biochemical/biophysical methods to characterize a variety of dystrophin and utrophin protein constructs in combination with complementary experiments in isolated cells and transgenic mice. The proposed studies will address several fundamental questions about the function of dystrophin in normal skeletal muscle and how its absence or abnormality causes human diseases of skeletal muscle.

Public Health Relevance

Duchenne muscular dystrophy (DMD) afflicts 1 in 3,500 live-born males and is typically lethal by the third decade. No effective therapies are currently available for DMD. Dystrophin is the protein missing in patients with DMD, but its function in muscle is not fully understood. Through rigorous biochemical/biophysical characterization of full-length dystrophin and utrophin in combination with complementary experiments in isolated cells and transgenic mice, the proposed studies will elucidate the functions of dystrophin in normal muscle and how its absence causes muscular dystrophy. Thus, this project is highly relevant to understanding the pathological mechanism of Duchenne muscular dystrophy and for the development of effective therapies.

National Institute of Health (NIH)
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Research Project (R01)
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Skeletal Muscle and Exercise Physiology Study Section (SMEP)
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Nuckolls, Glen H
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University of Minnesota Twin Cities
Schools of Medicine
United States
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Vulin, Adeline; Wein, Nicolas; Strandjord, Dana M et al. (2014) The ZZ domain of dystrophin in DMD: making sense of missense mutations. Hum Mutat 35:257-64
Belanto, Joseph J; Mader, Tara L; Eckhoff, Michael D et al. (2014) Microtubule binding distinguishes dystrophin from utrophin. Proc Natl Acad Sci U S A 111:5723-8
Johnson, Eric K; Li, Bin; Yoon, Jung Hae et al. (2013) Identification of new dystroglycan complexes in skeletal muscle. PLoS One 8:e73224
Henderson, Davin M; Lin, Ava Yun; Thomas, David D et al. (2012) The carboxy-terminal third of dystrophin enhances actin binding activity. J Mol Biol 416:414-24
Lin, Ava Yun; Prochniewicz, Ewa; Henderson, Davin M et al. (2012) Impacts of dystrophin and utrophin domains on actin structural dynamics: implications for therapeutic design. J Mol Biol 420:87-98
Zhang, Duan-Sun; Piazza, Valeria; Perrin, Benjamin J et al. (2012) Multi-isotope imaging mass spectrometry reveals slow protein turnover in hair-cell stereocilia. Nature 481:520-4
Henderson, Davin M; Belanto, Joseph J; Li, Bin et al. (2011) Internal deletion compromises the stability of dystrophin. Hum Mol Genet 20:2955-63
Henderson, Davin M; Lee, Ann; Ervasti, James M (2010) Disease-causing missense mutations in actin binding domain 1 of dystrophin induce thermodynamic instability and protein aggregation. Proc Natl Acad Sci U S A 107:9632-7
Prochniewicz, Ewa; Henderson, Davin; Ervasti, James M et al. (2009) Dystrophin and utrophin have distinct effects on the structural dynamics of actin. Proc Natl Acad Sci U S A 106:7822-7
Sonnemann, Kevin J; Heun-Johnson, Hanke; Turner, Amy J et al. (2009) Functional substitution by TAT-utrophin in dystrophin-deficient mice. PLoS Med 6:e1000083

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