Obesity is a risk factor for several preventable diseases. In recent years, there has been a dramatic rise in the consumer use of dietary supplements for weight management. Raspberry ketone [4-(p-hydroxyphenyl)-2-butanone] is derived from the fruit of the red raspberry (Rubus idaeus) and has purported antiobesity properties. Despite the supplement's consumer popularity, raspberry ketone has not been systematically tested to determine effectiveness for weight loss. The overall objective of this project is to determine the mechanisms by which raspberry ketone decreases feeding and prevents the metabolic consequences of diet-induced obesity. The central hypothesis of this project that raspberry ketone has a dual action to suppress feeding by activation of transient receptor potential cation channel subfamily V member 1 (TRPV1) channels and to increase fat oxidation mediated by peroxisome proliferator- activated receptor (PPAR) alpha pathways. This proposal will use diet-induced obese (DIO) mice to investigate the acute and long-term effects of raspberry ketone on body weight homeostasis and metabolic signatures.
Aim 1 will determine the mechanism(s) by which raspberry ketone suppresses feeding. This will include caloric intake and meal patterns, gene expression of gastrointestinal and hypothalamic feeding-related signals, circulating hormones, and potential for hepatic and intestinal pathology.
Aim 2 will determine the mechanism(s) by which raspberry ketone influences metabolic outcomes. This will include measuring energy expenditure, thermoregulatory activity, hemodynamic parameters, and glucose homeostasis.
Aim 3 will determine the bioacessibility of raspberry ketone using a functional model of the human digestive system (TNO intestinal model) and in vivo animal model to determine the metabolism and bioavailability of raspberry ketone. The findings from these studies will identify the in vivo mechanisms of preventative actions of raspberry ketone on weight gain and other obesity-related outcomes. The proposed project is relevant to the overall NIH goal of enhancing translational research by providing critical mechanistic data on a commonly used dietary supplement.

Public Health Relevance

Raspberry ketone is a popular dietary supplement used for weight loss. There is, however, little evidence on the effectiveness of this compound. This project will investigate the antiobesity signature of raspberry ketone in diet-induced obese mice. This project will examine the preventative action of raspberry ketone on the central and peripheral factors involved in body weight homeostasis. This preclinical research is instrumental in understanding how raspberry ketone prevents weight gain and retains metabolic control in face of an environment that promotes obesity.

Agency
National Institute of Health (NIH)
Institute
National Center for Complementary & Alternative Medicine (NCCAM)
Type
Research Project (R01)
Project #
1R01AT008933-01A1
Application #
9282089
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Duffy, Linda C
Project Start
2017-05-01
Project End
2022-04-30
Budget Start
2017-05-01
Budget End
2018-04-30
Support Year
1
Fiscal Year
2017
Total Cost
Indirect Cost
Name
Rutgers University
Department
Veterinary Sciences
Type
Earth Sciences/Resources
DUNS #
001912864
City
Piscataway
State
NJ
Country
United States
Zip Code
08854