The vast majority of hearing and balance impairments are thought to be due to death of sensory hair cells, the receptor cells of the inner ear. These cells are unusually metabolically active and hypersensitive to damage from overstimulation, some therapeutic drugs and environmental toxins, and aging. However, there is enormous variability in structural and functional outcomes of these challenges to the inner ear among both humans and laboratory animals, and a large number of mutations either directly influence the viability of hair cells, or alter susceptibility to ototoxicity. The goals of the research program proposed herein are: A) to better define the cellular and molecular cascades that control hair cell death and survival following exposure to potentially ototoxic agents;B) to use the genetic potential of the zebrafish and the accessibility of the lateral line neuromasts to identify and characterize genes that influence the viability of hair cells when challenged by ototoxic agents;and C) to employ the lateral line system to rapidly screen for small molecules/drugs that protect against damage. Four groups of experiments are proposed: 1) We will molecularly characterize 5 already identified mutations that confer resistance to aminoglycoside antibiotics;2) We will identify distinct pathways resulting in hair cell death using chemical and genetic modifiers of hair cell death;3) We will screen for new genes and drugs that alter the response to aminoglycosides;and 4) We will determine to what degree our findings from the zebrafish lateral line system extend to mammalian systems. In the US, over 31 million adults have trouble hearing. Our research will reveal how the sound-sensing cells of the ear are damaged, identify genes that may underlie the variability in hearing loss, and find drugs that may help in its prevention.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
5R01DC005987-10
Application #
8288064
Study Section
Auditory System Study Section (AUD)
Program Officer
Freeman, Nancy
Project Start
2003-04-01
Project End
2013-06-30
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
10
Fiscal Year
2012
Total Cost
$515,851
Indirect Cost
$185,177
Name
University of Washington
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
Stawicki, Tamara M; Owens, Kelly N; Linbo, Tor et al. (2014) The zebrafish merovingian mutant reveals a role for pH regulation in hair cell toxicity and function. Dis Model Mech 7:847-56
Suli, Arminda; Guler, Ali D; Raible, David W et al. (2014) A targeted gene expression system using the tryptophan repressor in zebrafish shows no silencing in subsequent generations. Development 141:1167-74
Esterberg, Robert; Hailey, Dale W; Rubel, Edwin W et al. (2014) ER-mitochondrial calcium flow underlies vulnerability of mechanosensory hair cells to damage. J Neurosci 34:9703-19
Coffin, Allison B; Williamson, Kay L; Mamiya, Anna et al. (2013) Profiling drug-induced cell death pathways in the zebrafish lateral line. Apoptosis 18:393-408
Bhandiwad, Ashwin A; Zeddies, David G; Raible, David W et al. (2013) Auditory sensitivity of larval zebrafish (Danio rerio) measured using a behavioral prepulse inhibition assay. J Exp Biol 216:3504-13
Esterberg, Robert; Hailey, Dale W; Coffin, Allison B et al. (2013) Disruption of intracellular calcium regulation is integral to aminoglycoside-induced hair cell death. J Neurosci 33:7513-25
Ou, Henry C; Lin, Vincent; Rubel, Edwin W (2013) "In-bone" utricle cultures--a simplified, atraumatic technique for in situ cultures of the adult mouse (Mus musculus) utricle. Otol Neurotol 34:353-9
Thomas, Andrew J; Hailey, Dale W; Stawicki, Tamara M et al. (2013) Functional mechanotransduction is required for cisplatin-induced hair cell death in the zebrafish lateral line. J Neurosci 33:4405-14
Coffin, Allison B; Rubel, Edwin W; Raible, David W (2013) Bax, Bcl2, and p53 differentially regulate neomycin- and gentamicin-induced hair cell death in the zebrafish lateral line. J Assoc Res Otolaryngol 14:645-59
Esterberg, Robert; Coffin, Allison B; Ou, Henry et al. (2013) Fish in a Dish: Drug Discovery for Hearing Habilitation. Drug Discov Today Dis Models 10:

Showing the most recent 10 out of 25 publications