This project addresses development of novel analytical, computational and experimental methodologies to model and analyze light scattering in biological tissue. Elastic scattering is the dominant mechanism of light-tissue interaction, plays a fundamental role in all light transport processes, and has been widely employed to provide diagnostic information about tissue structure and composition. However, elastic scattering in such complex media as tissue has not been fully understood. Questions remain regarding the origins of scattering in tissue and the appropriate methods of interpreting scattering signals. Bridging this gap is the main objective of the project. We will focus on elastic light interactions for microscopy applications. This requires development of innovative computational and experimental solutions. The study will elucidate which cellular structures affect scattering signal and image formation, which morphological properties of cells can be studied optically and, equally importantly, which properties cannot be probed. In particular, the focus will be on sensing sub-diffractional, nanoscale properties of cells. We will develop a free, open-source, user-friendly finite-difference time-domain (FDTD) electromagnetic simulation software for use in biomedical light scattering applications. We will relate optical and ultrastructural properties of cells. Finally, using the computational and experimental methodologies developed in the course of the project, we will identify specific cellular origins of nanoarchitectural changes in early carcinogenesis.
Development of robust methods of analysis of light-tissue interaction will allow design of optical techniques for tissue diagnosis. In particular, the proposed work will facilitate development of a microscopic technique to identify some of the earliest alterations of cellular nanoarchitecture in carcinogenesis.
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|Black, Kvar C L; Sileika, Tadas S; Yi, Ji et al. (2014) Bacterial killing by light-triggered release of silver from biomimetic metal nanorods. Small 10:169-78|
|Cherkezyan, Lusik; Subramanian, Hariharan; Backman, Vadim (2014) What structural length scales can be detected by the spectral variance of a microscope image? Opt Lett 39:4290-3|
|Patel, Mihir; Gomes, Andrew; Ruderman, Sarah et al. (2014) Polarization gating spectroscopy of normal-appearing duodenal mucosa to detect pancreatic cancer. Gastrointest Endosc 80:786-93.e1-2|
|Yi, Ji; Wei, Qing; Liu, Wenzhong et al. (2013) Visible-light optical coherence tomography for retinal oximetry. Opt Lett 38:1796-8|
|Cherkezyan, L; Capoglu, I; Subramanian, H et al. (2013) Interferometric spectroscopy of scattered light can quantify the statistics of subdiffractional refractive-index fluctuations. Phys Rev Lett 111:033903|
|Capoglu, Ilker R; Taflove, Allen; Backman, Vadim (2013) Computation of tightly-focused laser beams in the FDTD method. Opt Express 21:87-101|
|Mutyal, Nikhil N; Radosevich, Andrew; Tiwari, Ashish K et al. (2013) Biological mechanisms underlying structural changes induced by colorectal field carcinogenesis measured with low-coherence enhanced backscattering (LEBS) spectroscopy. PLoS One 8:e57206|
|Yi, Ji; Radosevich, Andrew J; Rogers, Jeremy D et al. (2013) Can OCT be sensitive to nanoscale structural alterations in biological tissue? Opt Express 21:9043-59|
|Gomes, Andrew J; Backman, Vadim (2013) Algorithm for automated selection of application-specific fiber-optic reflectance probes. J Biomed Opt 18:27012|
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