The conformations and stabilities of loop structures and adjacent stem regions will be studied in series of oligodeoxyribonucleotides prepared chemically by """"""""block"""""""" condensation methods and isolated in a state of high chemical purity. The results are expected to aid in the understanding of the shape and properties of DNA cruciform structures and the structural elements in the genomes of single-stranded DNA viruses. The methods to be used consist of differential molar absorptivity analysis, measurement of molar absorptivity - temperature profiles, NMR, circular dichroism, calorimetry, and X-ray crystallography.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM011518-22
Application #
3268272
Study Section
Biochemistry Study Section (BIO)
Project Start
1974-05-01
Project End
1989-06-30
Budget Start
1986-07-01
Budget End
1987-06-30
Support Year
22
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Purdue University
Department
Type
Schools of Arts and Sciences
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907