Directional cell migration is essential for organism development, immune surveillance and wound repair, but is also associated with numerous pathologies including cardiovascular and neurodegenerative disorders as well as tumor metastasis. Paxillin is a scaffold protein that localizes to sites of cell-matrix interaction calle focal adhesions and we have previously shown that paxillin functions primarily as a hub for regulating Rho GTPase signaling to coordinate actin cytoskeleton remodeling and focal adhesion turnover to drive cell migration. Importantly, polarized trafficking of pro-migratory factors including integrins, through the secretory machinery to the cell's leading edge, is also required for efficient directional migration. However, the mechanism by which cell motility and polarized trafficking are integrated is not well understood. In this continuing application, we wil evaluate a novel role for paxillin as a fundamental regulator of the cell polarization machinery, through its ability to modulate microtubule function via control of alpha-tubulin acetylation. Specifically, we will use a combination of RNAi and mutant expression, biochemical assays, combined with fixed and real-time fluorescence imaging, to test the hypothesis that paxillin regulates microtubule acetylation via the deacetylase HDAC6 to control Golgi organization and positioning, as well as ER-Golgi-plasma membrane trafficking of proteins including integrins and the metalloproteinase MT1-MMP to regulate directional cell migration and invasion. We will examine the influence of environmental context on this signaling axis by comparing 2D with 1D/3D matrix models of cell polarity. Furthermore, these studies will be performed in both normal and cancer cells in order to identify ubiquitous versus cancer specific roles for paxillin signaling through HDAC6 in polarized trafficking and migration that, in turn, may facilitate the development of therapeutic intervention strategies for various cell migration-related disorders.
Cell migration is important for embryonic development, wound healing and immune surveillance. Disregulated migration also contributes various pathologies including cancer cell invasion and metastasis cardiovascular disease and various neurological defects. For cells to migrate efficiently they must develop front-rear polarity. However, our understanding of the signaling mechanisms coupling the basic cell migration machinery to the establishment of a polarized phenotype is incomplete. The proposed research will utilize complimentary 1D, 2D and 3D matrix model systems to explore a new role for the focal adhesion adapter protein, paxillin in the regulation of directed cell migration by coordinating microtubule acetylation and thereby the polarized trafficking and delivery of proteins to the plasma membrane.
|Deakin, Nicholas O; Turner, Christopher E (2014) Paxillin inhibits HDAC6 to regulate microtubule acetylation, Golgi structure, and polarized migration. J Cell Biol 206:395-413|
|Jones, Matthew C; Machida, Kazuya; Mayer, Bruce J et al. (2013) Paxillin kinase linker (PKL) regulates Vav2 signaling during cell spreading and migration. Mol Biol Cell 24:1882-94|
|Pignatelli, Jeanine; Tumbarello, David A; Schmidt, Ronald P et al. (2012) Hic-5 promotes invadopodia formation and invasion during TGF-ýý-induced epithelial-mesenchymal transition. J Cell Biol 197:421-37|
|Deakin, Nicholas O; Turner, Christopher E (2011) Distinct roles for paxillin and Hic-5 in regulating breast cancer cell morphology, invasion, and metastasis. Mol Biol Cell 22:327-41|
|Yu, Jianxin A; Foley, Fiona C; Amack, Jeffrey D et al. (2011) The cell adhesion-associated protein Git2 regulates morphogenetic movements during zebrafish embryonic development. Dev Biol 349:225-37|
|Yu, Jianxin A; Deakin, Nicholas O; Turner, Christopher E (2010) Emerging role of paxillin-PKL in regulation of cell adhesion, polarity and migration. Cell Adh Migr 4:342-7|
|Zouq, Nadia K; Keeble, James A; Lindsay, Jennefer et al. (2009) FAK engages multiple pathways to maintain survival of fibroblasts and epithelia: differential roles for paxillin and p130Cas. J Cell Sci 122:357-67|
|Yu, Jianxin A; Deakin, Nicholas O; Turner, Christopher E (2009) Paxillin-kinase-linker tyrosine phosphorylation regulates directional cell migration. Mol Biol Cell 20:4706-19|
|Deakin, Nicholas O; Turner, Christopher E (2008) Paxillin comes of age. J Cell Sci 121:2435-44|
|Dabiri, Ganary; Tumbarello, David A; Turner, Christopher E et al. (2008) Hic-5 promotes the hypertrophic scar myofibroblast phenotype by regulating the TGF-beta1 autocrine loop. J Invest Dermatol 128:2518-25|
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