Over 100 genetic diseases are known to affect mitochondrial activity making this one of the most common classes of human pathologies. Mitochondrial function requires the coordinated expression of ~1500 nuclear-encoded genes and 37 mtDNA-encoded genes. This genomic organization has two important implications: 1) mitochondrial function is a large mutational target, and 2) nuclear-mitochondrial interactions are central to normal physiological performance. The long-term goal of this research program is to use Drosophila as a model to dissect the genetic bases of nuclear-mitochondrial interactions that affect organismal fitness. The initial phase of this research has uncovered a striking epistatic interaction where a particular mtDNA in one nuclear background (Oregon R) produces a suite of highly compromised phenotypes. When this same mtDNA is placed on an alternative set of wild chromosomes, these phenotypes are restored to near-wild type levels. The traits affected are hallmarks of metabolic disease: delayed development, reduced fecundity, locomotion, COX activity, and bristle defects. The current proposal seeks to identify the locus or loci responsible for this nuclear-mitochondrial interaction, and dissect the function of these genes. There are three specific aims: 1) Genetically map nuclear factors that modify mtDNA performance using segregation, meiotic and deficiency mapping. We will test the hypothesis that all affected phenotypes are due to a single locus, vs. independent loci for each trait;2) Molecular characterization of the mapped nuclear loci using SNP-array mapping among F2 offspring, and analyses of existing and novel alleles to identify the nucleotide changes causing specific phenotypes and, 3) Tissue specific expression of the identified genes. We will document the tissue specific expression of the newly identified genes, and their impact on mitochondrial gene expression. We will manipulate tissue specific expression to test the hypothesis that this nuclear-mitochondrial interaction effect on phenotype is distinct in different tissues.
These Aims will identify the genetic bases of strong mito-nuclear epistatic interactions uncovered in the first phase of the research, and undertake new mechanistic studies of how the mitochondrial enzyme complexes function under specific genetic manipulations of nuclear and mtDNA encoded proteins. These experiments will provide fundamental information on how specific mutations in nuclear and mitochondrial genes interact to determine organismal performance. As these interactions are evolutionarily ancient and highly conserved, the dissection of genetic pathways in Drosophila will be very relevant to understanding this very common class of diseases in humans.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM067862-08
Application #
8118536
Study Section
Genetic Variation and Evolution Study Section (GVE)
Program Officer
Krasnewich, Donna M
Project Start
2004-08-01
Project End
2012-07-31
Budget Start
2011-08-01
Budget End
2012-07-31
Support Year
8
Fiscal Year
2011
Total Cost
$318,726
Indirect Cost
Name
Brown University
Department
Biology
Type
Schools of Medicine
DUNS #
001785542
City
Providence
State
RI
Country
United States
Zip Code
02912
Mossman, Jim A; Tross, Jennifer G; Jourjine, Nick A et al. (2017) Mitonuclear Interactions Mediate Transcriptional Responses to Hypoxia in Drosophila. Mol Biol Evol 34:447-466
Mossman, Jim A; Biancani, Leann M; Zhu, Chen-Tseh et al. (2016) Mitonuclear Epistasis for Development Time and Its Modification by Diet in Drosophila. Genetics 203:463-84
Mossman, Jim A; Tross, Jennifer G; Li, Nan et al. (2016) Mitochondrial-Nuclear Interactions Mediate Sex-Specific Transcriptional Profiles in Drosophila. Genetics 204:613-630
Holmbeck, Marissa A; Donner, Julia R; Villa-Cuesta, Eugenia et al. (2015) A Drosophila model for mito-nuclear diseases generated by an incompatible interaction between tRNA and tRNA synthetase. Dis Model Mech 8:843-54
Holmbeck, Marissa A; Rand, David M (2015) Dietary Fatty Acids and Temperature Modulate Mitochondrial Function and Longevity in Drosophila. J Gerontol A Biol Sci Med Sci 70:1343-54
Villa-Cuesta, Eugenia; Rand, David M (2015) Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila. J Vis Exp :
Wu, Hao; Wang, Chi; Wu, Zhijin (2015) PROPER: comprehensive power evaluation for differential expression using RNA-seq. Bioinformatics 31:233-41
Zhu, Chen-Tseh; Ingelmo, Paul; Rand, David M (2014) G×G×E for lifespan in Drosophila: mitochondrial, nuclear, and dietary interactions that modify longevity. PLoS Genet 10:e1004354
Villa-Cuesta, Eugenia; Holmbeck, Marissa A; Rand, David M (2014) Rapamycin increases mitochondrial efficiency by mtDNA-dependent reprogramming of mitochondrial metabolism in Drosophila. J Cell Sci 127:2282-90
Meiklejohn, Colin D; Holmbeck, Marissa A; Siddiq, Mohammad A et al. (2013) An Incompatibility between a mitochondrial tRNA and its nuclear-encoded tRNA synthetase compromises development and fitness in Drosophila. PLoS Genet 9:e1003238

Showing the most recent 10 out of 41 publications