Huntington's disease (HD) is an autosomal dominant progressive neurodegenerative disease leading to both cognitive as well as motor deficits, and severe disability. The disease is caused by an unstable CAG repeat expansion which leads to a polyglutamine stretch within the protein huntingtin. The toxic effects of mutant huntingtin result in transcriptional dysregulation as well as mitochondrial dysfunction and oxidative damage. There is a deficiency of PGC-1alpha, a transcriptional coactivator which controls mitochondrial biogenesis and expression of antioxidant enzymes. There is also a deficiency of SIRT3, which is a protein deacetylase whose expression within mitochondria is dependent on PGC-1alpha. We propose validating both PGC-1alpha and SIRT3 as therapeutic targets for the treatment of HD. We will determine whether crossing both R6/2 and KI-zQ175 mice with SIRT3 overexpressing mice will produce neuroprotective effects. We will administer nicotinamide riboside (NR) in the diet as a means of activating SIRT1 and SIRT3, and determine whether this produces neuroprotective effects in both the R6/2 and the BACHD transgenic mouse models of HD. Lastly we will determine whether administration of the panPPAR agonist fenofibrate, or the RXR agonist bexarotene either alone or in combination will increase PGC-1alpha expression and exert neuroprotective effects in R6/2 and BACHD transgenic mice. These studies will validate PGC-1alpha and SIRT3 as therapeutic targets, and will determine whether NR, fenofibrate and bexarotene are suitable for further preclinical development, and ultimately for clinical trials to establish efficacy as neuroprotective therapies for HD.

Public Health Relevance

The proposed studies will determine whether increasing the activity of PGC-1alpha, SIRT1 and SIRT3 will exert neuroprotective effects in transgenic mouse models of Huntington's disease (HD). We will determine whether genetically increasing SIRT3, or administration of nicotinamide riboside (NR) to increase brain and mitochondrial levels of NAD+, and activate SIRT1 and SIRT3 are valid therapeutic strategies for HD. Lastly we will examine the effects of fenofibrate and bexarotene as means of pharmacologically increasing expression of PGC-1alpha, and thereby producing neuroprotective effects in transgenic mouse models of HD.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS086746-05
Application #
9617805
Study Section
Cell Death in Neurodegeneration Study Section (CDIN)
Program Officer
Miller, Daniel L
Project Start
2015-02-15
Project End
2020-12-31
Budget Start
2019-01-01
Budget End
2020-12-31
Support Year
5
Fiscal Year
2019
Total Cost
Indirect Cost
Name
Weill Medical College of Cornell University
Department
Neurology
Type
Schools of Medicine
DUNS #
060217502
City
New York
State
NY
Country
United States
Zip Code
10065
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