Abstract

Idiopathic Pulmonary Fibrosis (IPF) is a chronic interstitial lung diseases characterized by pulmonary inflammation and fibrosis. The pathogenesis of IPF involves multiple cell-cell interactions, cross-talk of multiple signal transduction pathways and gene regulation. MicroRNA is a non-coding ~22-nucleotide small RNA implicated in many biological processes and diseases. MicroRNAs regulate ~ 1/3 of mRNAs. However, nothing is known regarding the role of miRNAs in IPF. We hypothesize that miRNAs are dys-regulated during the progression of IPF and that these miRNAs regulate genes responsible for the development of IPF. Using a miRNA microarray, we will perform microRNA microarray analyses of LTRC IPF samples. The identified miRNAs will be verified by real-time PCR. The cellular localizations of the selected microRNAs will be determined by in situ hybridization. This pilot study will provide the basis for further functional studies of miRNAs in IPF.

Public Health Relevance

The completion of this proposal will further our knowledge about the pathogenesis of IPF as well as provide a baseboard for the development of novel therapeutic strategies.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Small Research Grants (R03)
Project #
5R03HL095383-02
Application #
7837598
Study Section
Special Emphasis Panel (ZHL1-CSR-H (M1))
Program Officer
Reynolds, Herbert Y
Project Start
2009-05-11
Project End
2012-04-30
Budget Start
2010-05-01
Budget End
2012-04-30
Support Year
2
Fiscal Year
2010
Total Cost
$73,850
Indirect Cost

  Institution

Name
Oklahoma State University Stillwater
Department
Physiology
Type
Schools of Veterinary Medicine
DUNS #
049987720
City
Stillwater
State
OK
Country
United States
Zip Code
74078

  Publications

Huang, Chaoqun; Xiao, Xiao; Yang, Ye et al. (2017) MicroRNA-101 attenuates pulmonary fibrosis by inhibiting fibroblast proliferation and activation. J Biol Chem 292:16420-16439
Huang, Chaoqun; Yang, Ye; Liu, Lin (2015) Interaction of long noncoding RNAs and microRNAs in the pathogenesis of idiopathic pulmonary fibrosis. Physiol Genomics 47:463-9
Xiao, Xiao; Huang, Chaoqun; Zhao, Chunling et al. (2015) Regulation of myofibroblast differentiation by miR-424 during epithelial-to-mesenchymal transition. Arch Biochem Biophys 566:49-57
Wang, Yang; Huang, Chaoqun; Reddy Chintagari, Narendranath et al. (2013) miR-375 regulates rat alveolar epithelial cell trans-differentiation by inhibiting Wnt/?-catenin pathway. Nucleic Acids Res 41:3833-44
Weng, Tingting; Mishra, Amarjit; Guo, Yujie et al. (2012) Regulation of lung surfactant secretion by microRNA-150. Biochem Biophys Res Commun 422:586-9
Zhao, Chunling; Huang, Chaoqun; Weng, Tingting et al. (2012) Computational prediction of MicroRNAs targeting GABA receptors and experimental verification of miR-181, miR-216 and miR-203 targets in GABA-A receptor. BMC Res Notes 5:91
Bhaskaran, Manoj; Xi, Dong; Wang, Yang et al. (2012) Identification of microRNAs changed in the neonatal lungs in response to hyperoxia exposure. Physiol Genomics 44:970-80