Human and animal studies have shown that fetal alcohol exposure increases the incidence of iron deficiency in infancy. Fetal alcohol-related infant iron deficiency is of particular importance in fetal alcohol spectrum disorders (FASD) because it may serve as a mediating mechanism by which alcohol exposure disrupts normal development. Moreover, iron deficiency has been shown to exacerbate the effects of fetal alcohol exposure on growth and neurologic function. In a prospective, longitudinal cohort study, we found that maternal binge drinking was associated with a 70% increase in prevalence of iron deficiency anemia (IDA) at age 6.5 mo. We also found that infants with IDA were markedly more vulnerable to effects of fetal alcohol on growth. Moreover, alcohol exposure and iron status have been shown to affect growth and behavioral outcomes synergistically in a rat model and to affect similar outcomes in humans. The objective of our study is to identify the mechanisms by which fetal alcohol exposure disrupts fetal iron stores and consequently leads to growth and cognitive deficits. Our preliminary data suggest that that the prenatal alcohol-related increase in IDA is due to a reduction in fetal iron stores resulting from disrupted placental iron transfer. We hypothesize that fetal alcohol exposure disrupts placental iron transfer from mother to fetus via two mechanisms: (a) alterations in placental structure that impede transfer of iron to the fetus and (b) altered expression of iron transport proteins, leading to disruption of cellular transplacental iron transport. In the proposed study, we aim to test this hypothesis and to examine the degree to which alcohol-related iron deficiency mediates and/or moderates fetal alcohol effects on somatic growth and neurobehavioral outcomes. We will recruit 90 pregnant women to provide a final sample of 60 mothers-30 heavy drinkers, 30 abstainer/light drinkers-and their infants, we will obtain prospective data on maternal alcohol consumption, diet, and biomarkers of iron status three times during pregnancy, placental samples at delivery, biomarkers of infant iron status at 2 wk and 6.5 mo, and neurobehavioral outcomes at 6.5 mo. First, we will examine the relation of fetal alcohol exposure to (1) placental structural abnormalities that impede nutrient transfer from mother to fetus and (2) alterations in placental iron transport protein expression. Next, we will determine the degree to which alcohol-related placental structural abnormalities and/or disrupted cellular transplacental iron transport mediate effects of fetal alcohol exposure on accretion of fetal iron stores, adjusting for other factors known to affect placental function and iron status, such as maternal iron status, smoking, and other drug use and gestational age at delivery. We will then determine the degree to which diminished fetal iron stores mediate and/or moderate the effects of prenatal alcohol exposure on growth and neurobehavioral function at 6.5 mo postpartum. This study provides an unusual opportunity to test a mechanistic hypothesis regarding the development of FASD directly in human subjects. Identification of mechanisms through which fetal alcohol-related infant iron deficiency mediates developmental sequelae of prenatal alcohol exposure has the potential to transform how we conceptualize the neuropathologic consequences of fetal alcohol exposure. It will also provide foundational knowledge for the NIAAA Strategic Plan for Research goal to """"""""use the knowledge gained in uncovering target sites for ethanol's action,"""""""" such as iron homeostasis, """"""""to begin the development of potential therapeutic or preventive interventions"""""""" for FASD.
Despite four decades of research in fetal alcohol spectrum disorders (FASD), maternal alcohol consumption during pregnancy continues to be a major public health problem in the U.S. and worldwide. One important effect of fetal alcohol exposure is a marked increase in iron deficiency in infancy, which may mediate and/or exacerbate growth and neurobehavioral sequelae of prenatal alcohol exposure. Our hypothesis that fetal alcohol-related effects on the placenta may disrupt the accumulation of fetal iron stores suggests that alcohol exposure can lead to iron deficiency anemia in infancy even when the mother herself is iron sufficient. Findings from this research can provide critical information about the pathophysiology of FASD, which can, in turn, contribute to the implementation of previously not considered, novel maternal and newborn interventions that are targeted to the specific mechanisms that mediate these disorders. The NIAAA Strategic Plan for Research has identified uncovering target sites for ethanol's action including iron homeostasis on the embryogenic and fetal stages of life to begin the development of potential therapeutic or preventive interventions, as a key long- term public health goal.
|Mei, Meng; Zhai, Chao; Li, Xinzhi et al. (2017) Characterization of aromatic residue-controlled protein retention in the endoplasmic reticulum of Saccharomyces cerevisiae. J Biol Chem 292:20707-20719|
|Lu, Zhenghui; Wang, Qinhong; Jiang, Sijing et al. (2016) Truncation of the unique N-terminal domain improved the thermos-stability and specific activity of alkaline ?-amylase Amy703. Sci Rep 6:22465|
|Bai, Wenqin; Cao, Yufan; Liu, Jun et al. (2016) Improvement of alkalophilicity of an alkaline xylanase Xyn11A-LC from Bacillus sp. SN5 by random mutation and Glu135 saturation mutagenesis. BMC Biotechnol 16:77|
|Lu, Yihong; Fang, Cheng; Wang, Qinhong et al. (2016) High-level expression of improved thermo-stable alkaline xylanase variant in Pichia Pastoris through codon optimization, multiple gene insertion and high-density fermentation. Sci Rep 6:37869|
|Pei, Xue; Fan, Feiyu; Lin, Liangcai et al. (2015) Involvement of the adaptor protein 3 complex in lignocellulase secretion in Neurospora crassa revealed by comparative genomic screening. Biotechnol Biofuels 8:124|
|Cai, Pengli; Wang, Bang; Ji, Jingxiao et al. (2015) The putative cellodextrin transporter-like protein CLP1 is involved in cellulase induction in Neurospora crassa. J Biol Chem 290:788-96|
|Carter, R C; Jacobson, J L; Jacobson, S W (2014) Fertility treatments, maternal intelligence, and child cognition. BJOG 121:1652|