The sodium-activated K+ current (IKNa) is present in many neurons of the mammalian brain. Using wave- clamp experiments we will test the hypothesis that IKNa is a significant but overlooked delayed outward current responsible for action potential repolarization throughout the brain. Our preliminary results suggest that IKNa is by far the largest K+ current in action potential repolarization in mitral cells. The importance of IKNa in controlling excitability and repolarizing action potentials in the nervous system has been overlooked because the sodium channel blocker TTX is often used to study K+ currents in neurons; we found that TTX blocks the component of INaP that activates IKNa. Thus TTX blocks IKNa as a secondary consequence of its blocking INaP. We recently showed that IKNa was functionally coupled to one or more persistent sodium currents, (INaP). Hence, another aim of this proposal is to attempt to reveal the molecular identity of the one or more channels producing INaP which is functionally coupled to IKNa. Although INaP is thought to be the product of voltage gated sodium channels (VGSCs), the exact identity of the individual VGSC alpha and beta subunits which produce INaP in particular neuron types, has not been well established. INaP, is the major target of most antiepileptic drugs. In neocortex, the prominent INaP in layer V pyramidal neurons, augments excitability in neurons that comprise the neocortical output circuits which are involved in the spread of epileptic activity. Thus, revealing the molecular identify of INaP in layer 5 pyramidal neurons might lead to drugs that more specifically and effectively target INaP leading to better control of epilepsy. We will also evaluae the role of IKNa in that cell type and others to reveal the extent to which the interaction of IKNa and INap is important in regulating excitability. These studies may also provide a better understanding of other neurological diseases where increased activity of INaP may be a factor such as Amyotrophic Lateral Sclerosis and Familial Hemiplegic Migraine; additionally, blockade of INaP may be a useful treatment for Multiple Sclerosis.
Specific Aims are: 1. to attempt to determine the molecular identities of TTX-sensitive INaP in several neuronal types including layer 5 cortical pyramidal neurons; 2. to attempt to identify the INaP component(s) functionally linked to the sodium-activated potassium channel (IKNa); 3. to test the hypothesis that IKNa is a significant but overlooked factor in action potential repolarization in many neuronal types.

Public Health Relevance

The coupled system of sodium-activated potassium currents (IKNa) and persistent sodium currents (INaP) may be of major importance and previously overlooked. We will test the hypothesis that IKNa is a significant factor in repolarization of actin potentials in many neurons of the brain. In addition, an increased understanding of the molecular basis of INaP, is important for better understanding of the basis and treatment of Epilepsy, and may also provide a better understanding and treatment of other neurological disease such Amyotrophic Lateral Sclerosis, Familial Hemiplegic Migraine and Multiple Sclerosis.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Exploratory/Developmental Grants (R21)
Project #
5R21NS088611-02
Application #
8999026
Study Section
Biophysics of Neural Systems Study Section (BPNS)
Program Officer
Silberberg, Shai D
Project Start
2015-02-01
Project End
2017-01-31
Budget Start
2016-02-01
Budget End
2017-01-31
Support Year
2
Fiscal Year
2016
Total Cost
Indirect Cost
Name
Washington University
Department
Neurosciences
Type
Schools of Medicine
DUNS #
068552207
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
Geng, Yanyan; Ferreira, Juan J; Dzikunu, Victor et al. (2017) A genetic variant of the sperm-specific SLO3 K+ channel has altered pH and Ca2+ sensitivities. J Biol Chem 292:8978-8987
Budelli, Gonzalo; Sun, Qi; Ferreira, Juan et al. (2016) SLO2 Channels Are Inhibited by All Divalent Cations That Activate SLO1 K+ Channels. J Biol Chem 291:7347-56