Songbirds are a leading model of neurobiological research with wide implications for understanding issues of human health and disease. They are among the few organisms that have evolved vocal learning, a complex trait that provides the basis of spoken language acquisition in humans. Studies of the ontogeny of songbird vocalizations and the organization of the brain circuitry that controls song learning and production have provided unique opportunities for uncovering the neural bases of vocal learning. Songbird research has also contributed novel insights into a broad range of fundamental questions in neurobiology, such as behaviorally- regulated gene expression, sex dimorphisms and the effects of sex steroids on brain structure and function, photoperiodicity and the regulation of seasonal brain plasticity, the role of sleep in learning, the neuroendocrine regulation of reproductive and social behaviors, and neurogenesis and neuronal replacement in adulthood. To help understand how the song control circuitry and birdsong behavior are shaped by genetic mechanisms, a wide set of modern molecular and genomic resources have recently become available to songbird researchers through NIH-funded initiatives;such resources include normalized brain cDNA libraries, comprehensive annotated EST databases, microarrays, a BAC library and the completed the zebra finch genome. Such resources have been instrumental in the rapid identification of genes and gene families of neurobiological interest, the study of gene structure and regulatory domains, high- throughput analysis of gene regulation through molecular profiling studies, and comparative genomics across the major higher vertebrate groups. A key next step in making full use of these genomic resources and understanding how genes relate to brain function and behavior in songbirds is to map gene expression in the context of functional brain circuits. To achieve this goal, we propose a single Specific Aim, namely to generate a Gene Expression Brain Atlas of the Zebra Finch. Specifically we propose to map the brain expression of a large set of genes (~2,500) that are of key importance to songbird and avian brain researchers, in register with a histological atlas, and make the data available as a web-based resource.

Public Health Relevance

This resource will have an enormous impact on further establishing songbirds as a major model organism for neurobiological research, and substantiating their relevance for human health and disease, in particular the neural basis of speech and language learning and impairment. It will provide a vastly improved molecular characterization of the zebra finch brain, allow for neurotransmitter and receptor profiling of song nuclei, and reveal how aene regulation mav be linked to the sona svstem. singing behavior and vocal learning.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Resource-Related Research Projects (R24)
Project #
5R24GM092842-04
Application #
8444464
Study Section
Genomics, Computational Biology and Technology Study Section (GCAT)
Program Officer
Sesma, Michael A
Project Start
2010-04-01
Project End
2014-03-31
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
4
Fiscal Year
2013
Total Cost
$371,525
Indirect Cost
$130,275
Name
Oregon Health and Science University
Department
Other Basic Sciences
Type
Schools of Medicine
DUNS #
096997515
City
Portland
State
OR
Country
United States
Zip Code
97239
Mello, C V; Clayton, D F (2015) The opportunities and challenges of large-scale molecular approaches to songbird neurobiology. Neurosci Biobehav Rev 50:70-6
Lovell, Peter V; Wirthlin, Morgan; Carbone, Lucia et al. (2015) Response to Hron et al. Genome Biol 16:165
Lovell, P V; Kasimi, B; Carleton, J et al. (2015) Living without DAT: Loss and compensation of the dopamine transporter gene in sauropsids (birds and reptiles). Sci Rep 5:14093
Olson, Christopher R; Hodges, Lisa K; Mello, Claudio V (2015) Dynamic gene expression in the song system of zebra finches during the song learning period. Dev Neurobiol 75:1315-38
Mello, Claudio V (2014) The zebra finch, Taeniopygia guttata: an avian model for investigating the neurobiological basis of vocal learning. Cold Spring Harb Protoc 2014:1237-42
Wirthlin, Morgan; Lovell, Peter V; Jarvis, Erich D et al. (2014) Comparative genomics reveals molecular features unique to the songbird lineage. BMC Genomics 15:1082
Carleton, Julia B; Lovell, Peter V; McHugh, Anne et al. (2014) An optimized protocol for high-throughput in situ hybridization of zebra finch brain. Cold Spring Harb Protoc 2014:1249-58
Pfenning, Andreas R; Hara, Erina; Whitney, Osceola et al. (2014) Convergent transcriptional specializations in the brains of humans and song-learning birds. Science 346:1256846
Olson, Christopher R; Wirthlin, Morgan; Lovell, Peter V et al. (2014) Proper care, husbandry, and breeding guidelines for the zebra finch, Taeniopygia guttata. Cold Spring Harb Protoc 2014:1243-8
Roeske, Tina C; Scharff, Constance; Olson, Christopher R et al. (2014) Long-distance retinoid signaling in the zebra finch brain. PLoS One 9:e111722

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