Funds are requested to purchase a Leica Laser Microdissection System to be shared by the laboratories of eight faculty, and will be made available for occasional use by other members of the UT Southwestern faculty. The primary users group includes members of the following departments, Molecular Biology (4), Internal Medicine (2), Cell Biology (1) and Pathology (1) of whom NIH currently funds 6. Space for the microscope will be provide by the department of Internal Medicine and the administration of the grant and management of the instrument will be supervised by a cross departmental committee. ? ? Projects proposed by the current users group include the following studies: (1) Identification of genes involved in calcium signaling pathways in skeletal and cardiac muscle (Olson); (2) Identification of genes involved in early heart development (Olson); (3) Identification of genes differentially expressed along the length of the linear heart tube (Olson); (4) Define the genetic regulatory program associated with the developing somite (Garry); (5) Analyze the gene expression pattern from wild type or mutant mouse hearts lacking essential cardiac transcription factors (Srivastava); (6) Compare gene expression in the specialized smooth muscle of the ductus arteriosus to the adjacent aortic smooth muscle (Srivastava); (7) Identification of transcription factors that establish and maintain the developmental program specific for the exocrine pancreas (MacDonald); (8) Identification of regulatory genes that control salivary gland development (MacDonald); (9) Identification of specific regulatory genes during neuronal differentiation controlled by bHLH factors in the developing spinal cord (Johnson). ? ?

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10RR019137-01
Application #
6737772
Study Section
Special Emphasis Panel (ZRG1-CDF-4 (31))
Program Officer
Tingle, Marjorie
Project Start
2004-05-01
Project End
2005-04-30
Budget Start
2004-05-01
Budget End
2005-04-30
Support Year
1
Fiscal Year
2004
Total Cost
$150,605
Indirect Cost
Name
University of Texas Sw Medical Center Dallas
Department
Biochemistry
Type
Schools of Medicine
DUNS #
800771545
City
Dallas
State
TX
Country
United States
Zip Code
75390
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Hullinger, Thomas G; Montgomery, Rusty L; Seto, Anita G et al. (2012) Inhibition of miR-15 protects against cardiac ischemic injury. Circ Res 110:71-81
Montgomery, Rusty L; Hullinger, Thomas G; Semus, Hillary M et al. (2011) Therapeutic inhibition of miR-208a improves cardiac function and survival during heart failure. Circulation 124:1537-47