Molecular biophysics at the University of Pennsylvania has a long and rich history. Eldridge Reeves Johnson, the former president of the Victor Talking Machine Company (RCA), gave the University a generous endowment in 1927 to establish a foundation in medical physics. The Johnson Foundation (JF) was dedicated to the """"""""study and development of physical methods in the investigation of disease and in its cure"""""""". The JF was the first organization in this country dedicated to biophysics. Under the leadership of Detlev Bronk, investigators developed and expanded methods for recording action potentials in nerves, developed innovative techniques of oxygen electrode measurements, and explored new technologies such as ultrasound and electron microscopy. Under the leadership of Britton Chances optical methods were pioneered at Penn to study cell organelles and tissues. In addition, research in the JF mapped out much of the molecular machinery of respiration and energy metabolism. The JF was also instrumental in pioneering the use of nuclear magnetic resonance spectroscopy to visualize biochemical processes in intact tissues. The Johnson Foundation, now under the direction of P. Leslie Dutton, has continued to evolve and expand into structural and computational biology, high resolution NMR spectroscopy, and protein folding. True to the mandate of the Johnson Foundation, biophysical methodologies are being used to explore fundamental processes that are vital to human biology and disease.

Public Health Relevance

The training program in Structural Biology and Molecular Biophysics at the University of Pennsylvania promotes the application of biophysics to clinically relevant research. The fundamental goal of our training mission is to produce biophysicists who can effectively integrate and apply the physical methodologies to medically relevant research problems. Our program explicitly integrates human biology and pathology into the graduate curriculum. The SBMB training program is designed to produce well-rounded scientists with expertise in structural biology and biophysics as well as have a solid foundation biomedical sciences and disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Institutional National Research Service Award (T32)
Project #
5T32GM008275-25
Application #
8496047
Study Section
Special Emphasis Panel (ZGM1-BRT-X (TG))
Program Officer
Flicker, Paula F
Project Start
1988-09-30
Project End
2014-06-30
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
25
Fiscal Year
2013
Total Cost
$275,523
Indirect Cost
$16,980
Name
University of Pennsylvania
Department
Biochemistry
Type
Schools of Medicine
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
McCullough, Cheryl E; Marmorstein, Ronen (2016) Molecular Basis for Histone Acetyltransferase Regulation by Binding Partners, Associated Domains, and Autoacetylation. ACS Chem Biol 11:632-42
Karch, K R; Sidoli, S; Garcia, B A (2016) Identification and Quantification of Histone PTMs Using High-Resolution Mass Spectrometry. Methods Enzymol 574:3-29
Moser, C C; Sheehan, M M; Ennist, N M et al. (2016) De Novo Construction of Redox Active Proteins. Methods Enzymol 580:365-88
Stetz, Matthew A; Carter, Marie V; Wand, A Joshua (2016) Optimized expression and purification of biophysical quantities of Lac repressor and Lac repressor regulatory domain. Protein Expr Purif 123:75-82
Smoak, Evan M; Stein, Paula; Schultz, Richard M et al. (2016) Long-Term Retention of CENP-A Nucleosomes in Mammalian Oocytes Underpins Transgenerational Inheritance of Centromere Identity. Curr Biol 26:1110-6
McCullough, C E; Marmorstein, R (2016) In Vitro Activity Assays for MYST Histone Acetyltransferases and Adaptation for High-Throughput Inhibitor Screening. Methods Enzymol 573:139-60
Bradley, Ryan P; Radhakrishnan, Ravi (2016) Curvature-undulation coupling as a basis for curvature sensing and generation in bilayer membranes. Proc Natl Acad Sci U S A 113:E5117-24
O'Brien, Evan S; Wand, A Joshua; Sharp, Kim A (2016) On the ability of molecular dynamics force fields to recapitulate NMR derived protein side chain order parameters. Protein Sci 25:1156-60
Xiao, Qi; Rubien, Jack D; Wang, Zhichun et al. (2016) Self-Sorting and Coassembly of Fluorinated, Hydrogenated, and Hybrid Janus Dendrimers into Dendrimersomes. J Am Chem Soc 138:12655-63
Lippert, Lisa G; Hallock, Jeffrey T; Dadosh, Tali et al. (2016) NeutrAvidin Functionalization of CdSe/CdS Quantum Nanorods and Quantification of Biotin Binding Sites using Biotin-4-Fluorescein Fluorescence Quenching. Bioconjug Chem 27:562-8

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