This application requests renewed support for an institutional postdoctoral training program in epilepsy and neuroscience that will allow faculty in the Departments of Biological Sciences, Molecular and Cellular Physiology, Comparative Medicine, Neurology and Neurological Sciences, Neurobiology, and Psychiatry at Stanford University to attract fellows to careers in research areas ultimately relevant to the problems of epilepsy in man. The faculties employ the methods of cellular neurophysiology and biophysics, live imaging, biochemistry, molecular biology, neuroanatomy, neuropharmacology, and the use of animal model systems for studies of normal and abnormal structure/function. Faculty research interests include cortical neuronal and glial development;physiological and morphological changes in nerve cells and circuits in animal models of chronic neocortical and hippocampal epileptogenesis;effects of drugs and transmitters on neurons and network activity in cortex and thalamus;development, organization, and synaptic physiology of the CNS, especially neocortex and hippocampus;cellular and molecular aspects of long-term changes in neuronal excitability;and the roles of gene structure, expression and modulation on neuronal function. Trainees may learn techniques of neurophysiology and neuropharmacology applied to neurons in slices or cultures, including patch-clamp methods for biophysical studies and assessment of transmitter actions;use of in vivo and in vitro animal models of epileptogenesis;anatomic techniques for intracellular labeling, immunocytochemistry and in situ hybridization;cell culture;molecular techniques including gene isolation and cloning, site directed mutagenesis;clonal analysis;cell transplantation;experimental gene therapy;and use of transgenic animals. A variety of available courses and seminars contribute to the breadth of research training. Participation of clinical department faculty fosters effective research interactions between MD and PhD trainees and a focus on the interface between basic neuroscience and clinical issues requiring investigation. The positions are advertised nationally and applicants solicited in accord with, and in the spirit of recruiting individuals from diverse backgrounds.

Public Health Relevance

The proposed training is designed to prepare future leaders in the field of Epilepsy Research. It will take place in laboratories of faculty within the Departments of Biology, Molecular and Cellular Physiology, Comparative Medicine, Neurobiology, Neurology and Psychiatry at Stanford University. Trainees will have broad exposure to relevant and timely issues including problems of epilepsy in man and application of modern neuroscience methods towards understanding seizures and development of new treatments.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Institutional National Research Service Award (T32)
Project #
5T32NS007280-28
Application #
8461997
Study Section
Special Emphasis Panel (ZNS1-SRB-P (56))
Program Officer
Korn, Stephen J
Project Start
1985-07-01
Project End
2016-06-30
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
28
Fiscal Year
2013
Total Cost
$228,671
Indirect Cost
$16,939
Name
Stanford University
Department
Neurology
Type
Schools of Medicine
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305
Fogerson, P Michelle; Huguenard, John R (2016) Tapping the Brakes: Cellular and Synaptic Mechanisms that Regulate Thalamic Oscillations. Neuron 92:687-704
DeNardo, Laura A; Berns, Dominic S; DeLoach, Katherine et al. (2015) Connectivity of mouse somatosensory and prefrontal cortex examined with trans-synaptic tracing. Nat Neurosci 18:1687-97
Makinson, Christopher D; Huguenard, John R (2015) Attentional flexibility in the thalamus: now we're getting SOMwhere. Nat Neurosci 18:2-4
Prince, David A (2014) How do we make models that are useful in understanding partial epilepsies? Adv Exp Med Biol 813:233-41
Yu, Xiaomeng M; Gutman, Itai; Mosca, Timothy J et al. (2013) Plum, an immunoglobulin superfamily protein, regulates axon pruning by facilitating TGF-ýý signaling. Neuron 78:456-68
Porter, Brenda E; Jacobson, Catherine (2013) Report of a parent survey of cannabidiol-enriched cannabis use in pediatric treatment-resistant epilepsy. Epilepsy Behav 29:574-7
Christian, Catherine A; Huguenard, John R (2013) Astrocytes potentiate GABAergic transmission in the thalamic reticular nucleus via endozepine signaling. Proc Natl Acad Sci U S A 110:20278-83
Christian, Catherine A; Huguenard, John R (2013) Sniffer patch laser uncaging response (SPLURgE): an assay of regional differences in allosteric receptor modulation and neurotransmitter clearance. J Neurophysiol 110:1722-31
Christian, Catherine A; Herbert, Anne G; Holt, Rebecca L et al. (2013) Endogenous positive allosteric modulation of GABA(A) receptors by diazepam binding inhibitor. Neuron 78:1063-74
Anderson, Garret R; Galfin, Timothy; Xu, Wei et al. (2012) Candidate autism gene screen identifies critical role for cell-adhesion molecule CASPR2 in dendritic arborization and spine development. Proc Natl Acad Sci U S A 109:18120-5

Showing the most recent 10 out of 34 publications