Abstract

This application proposes to apply high throughput biological discovery tools to identify and characterize genes and gene networks activated by pro- inflammatory, metabolic, and pathogen stresses affecting the cardiovascular system and the lung. The PGA features a highly interactive group of investigators with overlapping interests and unique technologies to contribute to the effort. A related and no less important objective of the applicants is the dissemination of tools and reagents to broadly enable academic investigators to study signal transduction pathways and gene expression patterns using tools and methods that until recently have been the exclusive province of the private sector. To fully exploit the opportunities these technologies offer, and to capitalize on the imminent completion of the sequencing of the human and mouse genomes, an integrated, multi-disciplinary research team has been assembled.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project--Cooperative Agreements (U01)
Project #
1U01HL066678-01
Application #
6292589
Study Section
Special Emphasis Panel (ZHL1-CSR-L (S2))
Project Start
2000-09-30
Project End
2004-07-31
Budget Start
2000-09-30
Budget End
2001-07-31
Support Year
1
Fiscal Year
2000
Total Cost
$3,490,121
Indirect Cost

  Institution

Name
Massachusetts General Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02199

  Publications

Wang, Xiaowei; Spandidos, Athanasia; Wang, Huajun et al. (2012) PrimerBank: a PCR primer database for quantitative gene expression analysis, 2012 update. Nucleic Acids Res 40:D1144-9
Spandidos, Athanasia; Wang, Xiaowei; Wang, Huajun et al. (2010) PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification. Nucleic Acids Res 38:D792-9
Liberati, Nicole T; Urbach, Jonathan M; Thurber, Tara K et al. (2008) Comparing insertion libraries in two Pseudomonas aeruginosa strains to assess gene essentiality. Methods Mol Biol 416:153-69
Spandidos, Athanasia; Wang, Xiaowei; Wang, Huajun et al. (2008) A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundance. BMC Genomics 9:633
Lee, Daniel G; Urbach, Jonathan M; Wu, Gang et al. (2006) Genomic analysis reveals that Pseudomonas aeruginosa virulence is combinatorial. Genome Biol 7:R90
Kulasakara, Hemantha; Lee, Vincent; Brencic, Anja et al. (2006) Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulence. Proc Natl Acad Sci U S A 103:2839-44
Kuo, Winston Patrick; Liu, Fang; Trimarchi, Jeff et al. (2006) A sequence-oriented comparison of gene expression measurements across different hybridization-based technologies. Nat Biotechnol 24:832-40
Salehi-Ashtiani, Kourosh; Luptak, Andrej; Litovchick, Alexander et al. (2006) A genomewide search for ribozymes reveals an HDV-like sequence in the human CPEB3 gene. Science 313:1788-92
Liberati, Nicole T; Urbach, Jonathan M; Miyata, Sachiko et al. (2006) An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc Natl Acad Sci U S A 103:2833-8
Lee, Mei-Ling Ting; Whitmore, G A; Bjorkbacka, Harry et al. (2005) Nonparametric methods for microarray data based on exchangeability and borrowed power. J Biopharm Stat 15:783-97

Showing the most recent 10 out of 23 publications