This application proposes to apply high throughput biological discovery tools to identify and characterize genes and gene networks activated by pro- inflammatory, metabolic, and pathogen stresses affecting the cardiovascular system and the lung. The PGA features a highly interactive group of investigators with overlapping interests and unique technologies to contribute to the effort. A related and no less important objective of the applicants is the dissemination of tools and reagents to broadly enable academic investigators to study signal transduction pathways and gene expression patterns using tools and methods that until recently have been the exclusive province of the private sector. To fully exploit the opportunities these technologies offer, and to capitalize on the imminent completion of the sequencing of the human and mouse genomes, an integrated, multi-disciplinary research team has been assembled.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project--Cooperative Agreements (U01)
Project #
5U01HL066678-02
Application #
6390936
Study Section
Special Emphasis Panel (ZHL1-CSR-L (S2))
Program Officer
Reinlib, Leslie
Project Start
2000-09-30
Project End
2004-07-31
Budget Start
2001-08-01
Budget End
2002-07-31
Support Year
2
Fiscal Year
2001
Total Cost
$3,460,723
Indirect Cost
Name
Massachusetts General Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02199
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Spandidos, Athanasia; Wang, Xiaowei; Wang, Huajun et al. (2010) PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification. Nucleic Acids Res 38:D792-9
Spandidos, Athanasia; Wang, Xiaowei; Wang, Huajun et al. (2008) A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundance. BMC Genomics 9:633
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