The mission of this Core is to provide state-of-art imaging technology and services to support the study: "Host-pathogen competition in IFN mediated antiviral defense." The proposed study incorporates skilled resources from more than four institutions addressing different but focused research questions involved in understanding the control and regulation of RIG-I- and MDA5-mediated immune surveillance during viral infection, with the overarching goal of developing novel therapeutic strategies for virus-associated disorders. As such, the Core currently features two major imaging systems: a spectral imaging confocal laser scanning microscope system equipped with live-cell imaging observation and a super-resolution molecule imaging system. All five projects will rely heavily on this Core, co-directed by Drs. Liang and Myong. What follows is a description of the confocal live-cell imaging portion of the Core followed by a description of the super-resolution single molecule microscopy services provided by the Core. All data from both systems are accessible to every PI. The data will be uploaded in the Center's website for data sharing and data mining. The amount of time supported varies over the 5-year course. The super-imaging system is more required in Projects 3 and 5, while the confocal system mainly supports Projects 1, 3, 4, and 5. In addition, the Imaging Core will coordinate the scientific and technologic services provided by each project.
The key to understand a protein's function is the accurate determination of its spatial/temporal distribution inside cells and its association with other relevant molecules. Using advanced technology provided by the imaging core to characterize RIG-1 performance will greatly facilitate our understanding of RIG-I during viral infection, thereby providing important insights for virus-infected diseases'control and theraphy.
|Rajsbaum, Ricardo; Garcia-Sastre, Adolfo; Versteeg, Gijs A (2014) TRIMmunity: the roles of the TRIM E3-ubiquitin ligase family in innate antiviral immunity. J Mol Biol 426:1265-84|
|Shi, Mude; Cho, Hyelim; Inn, Kyung-Soo et al. (2014) Negative regulation of NF-?B activity by brain-specific TRIpartite Motif protein 9. Nat Commun 5:4820|
|Peterson, Joseph R; Labhsetwar, Piyush; Ellermeier, Jeremy R et al. (2014) Towards a computational model of a methane producing archaeum. Archaea 2014:898453|
|Runge, Simon; Sparrer, Konstantin M J; Lässig, Charlotte et al. (2014) In vivo ligands of MDA5 and RIG-I in measles virus-infected cells. PLoS Pathog 10:e1004081|
|Deimling, Tobias; Cui, Sheng; Lammens, Katja et al. (2014) Crystal and solution structure of the human RIG-I SF2 domain. Acta Crystallogr F Struct Biol Commun 70:1027-31|
|Santiago, Felix W; Covaleda, Lina M; Sanchez-Aparicio, Maria T et al. (2014) Hijacking of RIG-I signaling proteins into virus-induced cytoplasmic structures correlates with the inhibition of type I interferon responses. J Virol 88:4572-85|
|Rajsbaum, Ricardo; Versteeg, Gijs A; Schmid, Sonja et al. (2014) Unanchored K48-linked polyubiquitin synthesized by the E3-ubiquitin ligase TRIM6 stimulates the interferon-IKK? kinase-mediated antiviral response. Immunity 40:880-95|
|Ayllon, Juan; Domingues, Patricia; Rajsbaum, Ricardo et al. (2014) A single amino acid substitution in the novel H7N9 influenza A virus NS1 protein increases CPSF30 binding and virulence. J Virol 88:12146-51|
|Roth, Susanne; Rottach, Andrea; Lotz-Havla, Amelie S et al. (2014) Rad50-CARD9 interactions link cytosolic DNA sensing to IL-1? production. Nat Immunol 15:538-45|
|Pulloor, Niyas Kudukkil; Nair, Sajith; McCaffrey, Kathleen et al. (2014) Human genome-wide RNAi screen identifies an essential role for inositol pyrophosphates in Type-I interferon response. PLoS Pathog 10:e1003981|
Showing the most recent 10 out of 103 publications