The importance ofthe Anopheles vector in malaria transmission is evident in the continued efforts to limit, modify, or eliminate populations of anophelines. In our epidemiologically diverse study sites (Peru, Brazil) we will focus on the molecular ecology ofthe most anthropophilic Neotropical malaria vector, Anopheles darlingi, and, identify additional local/regional vectors that our preliminary data suggest are important. We hypothesize that in the mining sites aroimd Puerto Maldonado in SE Peru, and in the new agricultural settlement, Remansinho, Acrelandia, as deforestation increases, so will the importance of An. darlingi in transmission and maintenance. This has not previously been carefully monitored, except along the Iquitos-Nauta HW, and we anticipate providing up-to-date risk assessment of each ofthe localities where we will work. The identification of adult female mosquitoes using standard keys is problematic, and PCR-ITS2-RFLP assays have been implemented on a local scale aroimd Iquitos. We will expand these assays to include our study sites in SE Peru and Acrelandia. Accurate species identification will provide information on changes in species composition over time, which also might alter risk of transmission. Using microsatellite markers that we have in hand (for An. darlingi) or will develop (for other local/regional vectors) we will test for changes in genotype, population structure and evidence of expansion, particularly in the most modified sites (mining, agriculture). An. darlingi is diverging into northem and southem lineages that we detected with the nuclear white gene. We developed a diagnostic PCR-RFLP assay to survey An. darlngi to determine the lineage distributions, because genetic differentiation could signify differences in vector competence, ecology or behavior. As bednets or ITNs become more acceptable, in some situations nets alone may provoke a behavioral change in the biting times of vector species, and the ITNs can influence levels of mosquito insecticide resistance. We will monitor peak biting times, biting rate, insecticide resistance, and changes in genetic diversity and effective population size to provide baseline data for evaluation, data that are currently unavailable in most ofthe Amazon. We will use a newly developed mosquito trap that reduces risk to collectors

Public Health Relevance

Through this research, we expect to provide basic data to: accurately incriminate the primary malaria vector species in three regions, discover the impact of ecological changes (deforestafion and mining) on their population structure and behavior, and the evaluate the usefulness of ITNs or insecticide treatment to control An. dariingi and other vectors.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI089681-03
Application #
8381268
Study Section
Special Emphasis Panel (ZAI1-AWA-M)
Project Start
2012-07-01
Project End
2017-06-30
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
3
Fiscal Year
2012
Total Cost
$300,155
Indirect Cost
$20,701
Name
University of California San Diego
Department
Type
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Rosas-Aguirre, Angel; Guzman-Guzman, Mitchel; Gamboa, Dionicia et al. (2017) Micro-heterogeneity of malaria transmission in the Peruvian Amazon: a baseline assessment underlying a population-based cohort study. Malar J 16:312
Carrasco-Escobar, Gabriel; Miranda-Alban, Julio; Fernandez-MiƱope, Carlos et al. (2017) High prevalence of very-low Plasmodium falciparum and Plasmodium vivax parasitaemia carriers in the Peruvian Amazon: insights into local and occupational mobility-related transmission. Malar J 16:415
Campos, Melina; Conn, Jan E; Alonso, Diego Peres et al. (2017) Microgeographical structure in the major Neotropical malaria vector Anopheles darlingi using microsatellites and SNP markers. Parasit Vectors 10:76
Sanchez, Juan F; Carnero, Andres M; Rivera, Esteban et al. (2017) Unstable Malaria Transmission in the Southern Peruvian Amazon and Its Association with Gold Mining, Madre de Dios, 2001-2012. Am J Trop Med Hyg 96:304-311
Moreno, Marta; Saavedra, Marlon P; Bickersmith, Sara A et al. (2017) Intensive trapping of blood-fed Anopheles darlingi in Amazonian Peru reveals unexpectedly high proportions of avian blood-meals. PLoS Negl Trop Dis 11:e0005337
Cowell, Annie N; Loy, Dorothy E; Sundararaman, Sesh A et al. (2017) Selective Whole-Genome Amplification Is a Robust Method That Enables Scalable Whole-Genome Sequencing of Plasmodium vivax from Unprocessed Clinical Samples. MBio 8:
Li, Fengwu; Bounkeua, Viengngeun; Pettersen, Kenneth et al. (2016) Plasmodium falciparum ookinete expression of plasmepsin VII and plasmepsin X. Malar J 15:111
Hupalo, Daniel N; Luo, Zunping; Melnikov, Alexandre et al. (2016) Population genomics studies identify signatures of global dispersal and drug resistance in Plasmodium vivax. Nat Genet 48:953-8
Rosas-Aguirre, Angel; Gamboa, Dionicia; Manrique, Paulo et al. (2016) Epidemiology of Plasmodium vivax Malaria in Peru. Am J Trop Med Hyg 95:133-144
Van Voorhis, Wesley C; Adams, John H; Adelfio, Roberto et al. (2016) Open Source Drug Discovery with the Malaria Box Compound Collection for Neglected Diseases and Beyond. PLoS Pathog 12:e1005763

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