Antigen-specific antibody quantification will be required for Influenza and Hepatitis B antigens that are vaccine components, as well as non-vaccine Influenza antigens to define cross-reactivity. Measurements for standardized hepatitis B vaccine antigens will be through medical service providers. However, a careful and thorough definition of influenza antigens is central to this proposal. The core will have to accurately quantify serum antibody titers against: i) Flu vaccine component antigens, ii) Flu non-vaccine component antigens, and iii) hemagglutinins from different Flu strains. This core will support the U19 program through four aims:
Aim 1 : Quantification of antigen-specific antibodies and antigen-specific B cells. The core will measure with high fidelity serum antibodies against Influenza antigens. This will support Project 1, 2, 3, 4, and 5.
Aim 2 : Characterization of anti-Influenza neutralizing antibodies. The core will score neutralizing anti- Influenza serum antibody levels and appraise their cross-reactivity. This will support Project 1, 2, 3, 4, and 5.
Aim 3 : Measurement of serum cytokine and chemokine levels. The core will measure the levels of multiple cytokines and chemokines in sera and culture supernatants. This will support Project 1,2,3, 4, and 5.
Aim 4 : Detection of antibodies against self antigens. The core will perform proteome-wide surveys of auto-antibodies for Project 3. This will support Project 4.
Aim 5 : Development of novel biomarker detection reagents. The core will develop novel protein and antibody-based biomarkers for validation of discoveries made through all the Project areas. This will support Project 1, 2, 3,4, and 5.

Public Health Relevance

This core will support the U19 program by accurately quantifying serum antibody titers against: i) Flu vaccine component antigens, ii) Flu non-vaccine component antigens, and iii) hemagglutinins from different Flu strains. It will also measure serum cytokines and develop novel biomarker detection methods.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Program--Cooperative Agreements (U19)
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Special Emphasis Panel (ZAI1-QV-I)
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Baylor Research Institute
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Hogstad, Brandon; Berres, Marie-Luise; Chakraborty, Rikhia et al. (2018) RAF/MEK/extracellular signal-related kinase pathway suppresses dendritic cell migration and traps dendritic cells in Langerhans cell histiocytosis lesions. J Exp Med 215:319-336
Cepika, Alma-Martina; Banchereau, Romain; Segura, Elodie et al. (2017) A multidimensional blood stimulation assay reveals immune alterations underlying systemic juvenile idiopathic arthritis. J Exp Med 214:3449-3466
Athale, Shruti; Banchereau, Romain; Thompson-Snipes, LuAnn et al. (2017) Influenza vaccines differentially regulate the interferon response in human dendritic cell subsets. Sci Transl Med 9:
Banchereau, Romain; Cepika, Alma-Martina; Banchereau, Jacques et al. (2017) Understanding Human Autoimmunity and Autoinflammation Through Transcriptomics. Annu Rev Immunol 35:337-370
Silvin, Aymeric; Yu, Chun I; Lahaye, Xavier et al. (2017) Constitutive resistance to viral infection in human CD141+ dendritic cells. Sci Immunol 2:
Schotsaert, Michael; García-Sastre, Adolfo (2017) Inactivated influenza virus vaccines: the future of TIV and QIV. Curr Opin Virol 23:102-106
HIPC-CHI Signatures Project Team; HIPC-I Consortium (2017) Multicohort analysis reveals baseline transcriptional predictors of influenza vaccination responses. Sci Immunol 2:
Bentebibel, Salah-Eddine; Khurana, Surender; Schmitt, Nathalie et al. (2016) ICOS(+)PD-1(+)CXCR3(+) T follicular helper cells contribute to the generation of high-avidity antibodies following influenza vaccination. Sci Rep 6:26494
Heinonen, Santtu; Jartti, Tuomas; Garcia, Carla et al. (2016) Rhinovirus Detection in Symptomatic and Asymptomatic Children: Value of Host Transcriptome Analysis. Am J Respir Crit Care Med 193:772-82
Sandoval, Carmen; Barrera, Aldo; Ferrés, Marcela et al. (2016) Infection in Health Personnel with High and Low Levels of Exposure in a Hospital Setting during the H1N1 2009 Influenza A Pandemic. PLoS One 11:e0147271

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