Abstract

Public Health Relevance

This project provides basic research on integral membrane proteins, which are found in all living organisms. This work contributes to the appreciation of biological processes and gives unique insight into how these proteins contribute to human health and wellbeing. Methods developed in this research may also lead to better treatments for diseases.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54GM094584-02
Application #
8306881
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2011-07-01
Budget End
2012-06-30
Support Year
2
Fiscal Year
2011
Total Cost
$929,575
Indirect Cost

  Institution

Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715

  Publications

Aoki, Scott T; Porter, Douglas F; Prasad, Aman et al. (2018) An RNA-Binding Multimer Specifies Nematode Sperm Fate. Cell Rep 23:3769-3775
Minkoff, Benjamin B; Makino, Shin-Ichi; Haruta, Miyoshi et al. (2017) A cell-free method for expressing and reconstituting membrane proteins enables functional characterization of the plant receptor-like protein kinase FERONIA. J Biol Chem 292:5932-5942
Alderson, Thomas Reid; Kim, Jin Hae; Markley, John Lute (2016) Dynamical Structures of Hsp70 and Hsp70-Hsp40 Complexes. Structure 24:1014-30
Li, Bo; Makino, Shin-Ichi; Beebe, Emily T et al. (2016) Cell-free translation and purification of Arabidopsis thaliana regulator of G signaling 1 protein. Protein Expr Purif 126:33-41
Lee, Kanghyun; Sharma, Ruchika; Shrestha, Om Kumar et al. (2016) Dual interaction of the Hsp70 J-protein cochaperone Zuotin with the 40S and 60S ribosomal subunits. Nat Struct Mol Biol 23:1003-1010
Stefely, Jonathan A; Licitra, Floriana; Laredj, Leila et al. (2016) Cerebellar Ataxia and Coenzyme Q Deficiency through Loss of Unorthodox Kinase Activity. Mol Cell 63:608-620
Aoki, Scott T; Kershner, Aaron M; Bingman, Craig A et al. (2016) PGL germ granule assembly protein is a base-specific, single-stranded RNase. Proc Natl Acad Sci U S A 113:1279-84
Aceti, David J; Bingman, Craig A; Wrobel, Russell L et al. (2015) Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies. J Struct Funct Genomics 16:67-80
Bai, Yonghong; McCoy, Jason G; Levin, Elena J et al. (2015) X-ray structure of a mammalian stearoyl-CoA desaturase. Nature 524:252-6
Beebe, Emily T; Makino, Shin-Ichi; Markley, John L et al. (2014) Automated cell-free protein production methods for structural studies. Methods Mol Biol 1140:117-35

Showing the most recent 10 out of 19 publications