Cell and Tissue Core Introduction to revised application: We appreciate the study section recommending approval this Core with an excellent/outstanding descriptor. The following concerns or suggestions were described by the reviewers: Transport of tissue from the Vanderbilt Center to Yale: As suggested by reviewer 1, """"""""this has likely been solved."""""""" As described in the revised description of the mouse modeling core, Dr. Osteen and fellow investigators have routinely shipped tissue and other material to collaborators throughout the world. Overnight shipping of frozen material on dry ice has been used to deliver tissue from Vanderbilt to Yale as part of ongoing collaborations between the groups. Development of non-radioactive in situ hybridization : Reviewer one suggested that """"""""future development should work towards expertise in non-radioactive in situ hybridization."""""""" We agree that this would complement our existing techniques. We have included a plan to provide this technology and incorporate it into the center by the end of year one. The expertise is already present in the Center as demonstrated in preliminary data presented in Project I. Budget. Reviewer two correctly pointed out that we did not justify the projected 50% saving from use of the core. These savings come in the form of bulk purchases and eliminating redundant experiments and tissue collection. As all investigators in the Center will use endometrial tissue and most will perform similar experiments using cultured cells, we estimate that the cost of a single technician (rather than the duplicated efforts of two or three) will account for at least a 50% cost reduction. Long-term freezing and storage. Reviewer two suggested the use of a cryogenic system reaching temperatures below -130?C for long term storage of cells. We do indeed have access to a Yale University freezing facility operated by the Cancer Center. This facility will allow storage of cells on a fee for service basis. This facility is subsidized by the University and is more cost effective than establishing our own facility. We apologize for not including this information in the original application. We thank the study section for the constructive criticism. All of the requested information is now provided and all suggestions incorporated into the revised application.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54HD052668-04
Application #
8065990
Study Section
Special Emphasis Panel (ZHD1)
Project Start
Project End
Budget Start
2010-04-01
Budget End
2011-03-31
Support Year
4
Fiscal Year
2010
Total Cost
$265,914
Indirect Cost
Name
Yale University
Department
Type
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
Liu, Ying; Tal, Reshef; Pluchino, Nicola et al. (2018) Systemic administration of bone marrow-derived cells leads to better uterine engraftment than use of uterine-derived cells or local injection. J Cell Mol Med 22:67-76
Flannery, Clare A; Choe, Gina H; Cooke, Katherine M et al. (2018) Insulin Regulates Glycogen Synthesis in Human Endometrial Glands Through Increased GYS2. J Clin Endocrinol Metab 103:2843-2850
Sahin, Cagdas; Mamillapalli, Ramanaiah; Yi, Kyong W et al. (2018) microRNA Let-7b: A Novel treatment for endometriosis. J Cell Mol Med 22:5346-5353
Pluchino, Nicola; Mamillapalli, Ramanaiah; Moridi, Irene et al. (2018) G-Protein-Coupled Receptor CXCR7 Is Overexpressed in Human and Murine Endometriosis. Reprod Sci 25:1168-1174
Sahin, Cagdas; Mamillapalli, Ramanaiah; Taylor, Hugh S (2018) Bone Marrow-Derived Cells Trafficking to the Oviduct: Effect of Ischemia-Reperfusion Injury. Reprod Sci 25:1037-1044
Moridi, Irene; Mamillapalli, Ramanaiah; Cosar, Emine et al. (2017) Bone Marrow Stem Cell Chemotactic Activity Is Induced by Elevated CXCl12 in Endometriosis. Reprod Sci 24:526-533
Naqvi, Hanyia; Mamillapalli, Ramanaiah; Krikun, Graciela et al. (2016) Endometriosis Located Proximal to or Remote From the Uterus Differentially Affects Uterine Gene Expression. Reprod Sci 23:186-91
Flannery, Clare A; Saleh, Farrah L; Choe, Gina H et al. (2016) Differential Expression of IR-A, IR-B and IGF-1R in Endometrial Physiology and Distinct Signature in Adenocarcinoma. J Clin Endocrinol Metab 101:2883-91
Flannery, Clare A; Fleming, Andrew G; Choe, Gina H et al. (2016) Endometrial Cancer-Associated FGF18 Expression Is Reduced by Bazedoxifene in Human Endometrial Stromal Cells In Vitro and in Murine Endometrium. Endocrinology 157:3699-3708
Kulp, Jennifer L; Mamillapalli, Ramanaiah; Taylor, Hugh S (2016) Aberrant HOXA10 Methylation in Patients With Common Gynecologic Disorders: Implications for Reproductive Outcomes. Reprod Sci 23:455-63

Showing the most recent 10 out of 83 publications