-We have established a treatment that allows us to efficiently and conditionally immortalize primary human keratinocytes and other epithelial cells by culturing them in the presence of a ROCK inhibitor and with feeder fibroblasts. -The immortalized cells are invaluable for studies on the long-term persistent HPV DNA replication -Immortalization is conditional -The immortalized cells are invaluable for studies on gene therapy and gene delivery. -The technique is invaluable for the efficient procurement of cells from individuals with specific genetic and infectious diseases.
|Chapman, Sandra; McDermott, David H; Shen, Kui et al. (2014) The effect of Rho kinase inhibition on long-term keratinocyte proliferation is rapid and conditional. Stem Cell Res Ther 5:60|
|Liu, Xuefeng; Ory, Virginie; Chapman, Sandra et al. (2012) ROCK inhibitor and feeder cells induce the conditional reprogramming of epithelial cells. Am J Pathol 180:599-607|
|Buck, Christopher B; Phan, Giao Q; Raiji, Manish T et al. (2012) Complete genome sequence of a tenth human polyomavirus. J Virol 86:10887|
|Jordan, Catherine T; Cao, Li; Roberson, Elisha D O et al. (2012) PSORS2 is due to mutations in CARD14. Am J Hum Genet 90:784-95|
|Chapman, Sandra; Liu, Xuefeng; Meyers, Craig et al. (2010) Human keratinocytes are efficiently immortalized by a Rho kinase inhibitor. J Clin Invest 120:2619-26|