-We have established a treatment that allows us to efficiently and conditionally immortalize primary human keratinocytes and other epithelial cells by culturing them in the presence of a ROCK inhibitor and with feeder fibroblasts. -The immortalized cells are invaluable for studies on the long-term persistent HPV DNA replication -Immortalization is conditional -The immortalized cells are invaluable for studies on gene therapy and gene delivery. -The technique is invaluable for the efficient procurement of cells from individuals with specific genetic and infectious diseases.

Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
2013
Total Cost
$241,539
Indirect Cost
City
State
Country
Zip Code
Chapman, Sandra; McDermott, David H; Shen, Kui et al. (2014) The effect of Rho kinase inhibition on long-term keratinocyte proliferation is rapid and conditional. Stem Cell Res Ther 5:60