We have previously established a treatment that allows us to efficiently and conditionally immortalize primary human keratinocytes and other epithelial cells by culturing them in the presence of a ROCK inhibitor and with feeder fibroblasts. The technique is invaluable for the efficient procurement of cells from individuals with specific genetic and infectious diseases. In FY2019 we have isolated keratinocytes and fibroblasts from individuals at the NIH clinical center and extramural centers to assess the role of immunodeficiency in HPV infection and associated cancer.
| Stepp, Wesley H; Stamos, James D; Khurana, Simran et al. (2017) Sp100 colocalizes with HPV replication foci and restricts the productive stage of the infectious cycle. PLoS Pathog 13:e1006660 |
| Chapman, Sandra; McDermott, David H; Shen, Kui et al. (2014) The effect of Rho kinase inhibition on long-term keratinocyte proliferation is rapid and conditional. Stem Cell Res Ther 5:60 |
| Buck, Christopher B; Phan, Giao Q; Raiji, Manish T et al. (2012) Complete genome sequence of a tenth human polyomavirus. J Virol 86:10887 |
| Jordan, Catherine T; Cao, Li; Roberson, Elisha D O et al. (2012) PSORS2 is due to mutations in CARD14. Am J Hum Genet 90:784-95 |
| Liu, Xuefeng; Ory, Virginie; Chapman, Sandra et al. (2012) ROCK inhibitor and feeder cells induce the conditional reprogramming of epithelial cells. Am J Pathol 180:599-607 |
| Chapman, Sandra; Liu, Xuefeng; Meyers, Craig et al. (2010) Human keratinocytes are efficiently immortalized by a Rho kinase inhibitor. J Clin Invest 120:2619-26 |
