Utilization of a very small anomalous diffraction signal provided by sulfur atoms of the cysteine and methionine residues or by phosphorus atoms of nucleic acids, intrinsically present in all proteins or RNA/DNA can be successfully used to solve crystal structures of proteins. This approach, in part pioneered by us, is currently gaining increasing popularity and becomes a routine technique in the practice of macromolecular crystallography. The diffraction data to extremely high resolution in the range of 0.55-0.7 angstrom were measured using synchrotron radiation from crystals of oligonucleotides and oligopeptides, and the refined models revealeed the unprecedented structural details of the investigated molecules including electron densities at the sigma bonds between atoms. The observed very fine features in this structures will serve to improve the stereochemical libraries used for routine refinement of protein crystal structures at lower resolution. We participated in several collaborative structural projects on various biologically important proteins, such as fluorescenty proteins used as biomarkers, human mitochindrial helicase, rhizobium fucosyltransferase and plant S-adenosyl-homocysteine hydrolase.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIABC010378-14
Application #
8763087
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
14
Fiscal Year
2013
Total Cost
$1,400,310
Indirect Cost
Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code
Dauter, Zbigniew; Wlodawer, Alexander (2016) Progress in protein crystallography. Protein Pept Lett 23:201-10
Xu, Beibei; Luo, Zhipu; Wilson, Andrew J et al. (2016) Multifunctional Charge-Transfer Single Crystals through Supramolecular Assembly. Adv Mater 28:5322-9
Dauter, Zbigniew (2016) Objective evaluation of radiation damage in a nucleoprotein complex. Acta Crystallogr D Struct Biol 72:601-2
Kiliszek, Agnieszka; Banaszak, Katarzyna; Dauter, Zbigniew et al. (2016) The first crystal structures of RNA-PNA duplexes and a PNA-PNA duplex containing mismatches--toward anti-sense therapy against TREDs. Nucleic Acids Res 44:1937-43
Ruszkowski, Milosz; Dauter, Zbigniew (2016) Structural Studies of Medicago truncatula Histidinol Phosphate Phosphatase from Inositol Monophosphatase Superfamily Reveal Details of Penultimate Step of Histidine Biosynthesis in Plants. J Biol Chem 291:9960-73
Minor, Wladek; Dauter, Zbigniew; Helliwell, John R et al. (2016) Safeguarding Structural Data Repositories against Bad Apples. Structure 24:216-20
Malinska, Maura; Dauter, Zbigniew (2016) Transferable aspherical atom model refinement of protein and DNA structures against ultrahigh-resolution X-ray data. Acta Crystallogr D Struct Biol 72:770-9
Sliwiak, Joanna; Dauter, Zbigniew; Jaskolski, Mariusz (2016) Crystal Structure of Hyp-1, a Hypericum perforatum PR-10 Protein, in Complex with Melatonin. Front Plant Sci 7:668
Sekula, Bartosz; Ruszkowski, Milosz; Malinska, Maura et al. (2016) Structural Investigations of N-carbamoylputrescine Amidohydrolase from Medicago truncatula: Insights into the Ultimate Step of Putrescine Biosynthesis in Plants. Front Plant Sci 7:350
Dauter, Zbigniew (2015) Solving coiled-coil protein structures. IUCrJ 2:164-5

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