The purpose of the current work is to understand the signaling function, biology, and physiology of the evolutionarily-conserved Gbeta5/R7-RGS/R7BP complex in humans. To this end we are first trying to identify and characterize the functions of the Gbeta5 complex in model systems such as cultured PC12 and SH-SY5Y cells, primary rodent brain neurons, and genetically-modified mice. The effect of the loss-of-function of Gbeta5 and other complex components in knockout mice is currently being examined using developmental, behavioral, immunohistochemical, and neuroanatomical analyses.

Project Start
Project End
Budget Start
Budget End
Support Year
20
Fiscal Year
2013
Total Cost
$557,791
Indirect Cost
City
State
Country
Zip Code
Zhang, Jian-Hua; Adikaram, Poorni; Pandey, Mritunjay et al. (2016) Optimization of genome editing through CRISPR-Cas9 engineering. Bioengineered 7:166-74
Zhang, Jian-Hua; Pandey, Mritunjay; Seigneur, Erica M et al. (2011) Knockout of G protein β5 impairs brain development and causes multiple neurologic abnormalities in mice. J Neurochem 119:544-54
Panicker, Leelamma M; Zhang, Jian-Hua; Posokhova, Ekaterina et al. (2010) Nuclear localization of the G protein beta 5/R7-regulator of G protein signaling protein complex is dependent on R7 binding protein. J Neurochem 113:1101-12