The purpose of the current work is to understand the signaling function, biology, and physiology of the evolutionarily-conserved Gbeta5/R7-RGS/R7BP complex in humans. To this end we are first trying to identify and characterize the functions of the Gbeta5 complex in model systems such as cultured PC12 and SH-SY5Y cells, primary rodent brain neurons, and genetically-modified mice. The effect of the loss-of-function of Gbeta5 and other complex components in knockout mice is currently being examined using developmental, behavioral, immunohistochemical, and neuroanatomical analyses.
|Pandey, Mritunjay; Zhang, Jian-Hua; Mishra, Santosh K et al. (2017) A central role for R7bp in the regulation of itch sensation. Pain 158:931-944|
|Zhang, Jian-Hua; Adikaram, Poorni; Pandey, Mritunjay et al. (2016) Optimization of genome editing through CRISPR-Cas9 engineering. Bioengineered 7:166-74|
|Lodder, Elisabeth M; De Nittis, Pasquelena; Koopman, Charlotte D et al. (2016) GNB5 Mutations Cause an Autosomal-Recessive Multisystem Syndrome with Sinus Bradycardia and Cognitive Disability. Am J Hum Genet 99:786|
|Nini, Lylia; Zhang, Jian-Hua; Pandey, Mritunjay et al. (2012) Expression of the G?5/R7-RGS protein complex in pituitary and pancreatic islet cells. Endocrine 42:214-7|
|Zhang, Jian-Hua; Pandey, Mritunjay; Seigneur, Erica M et al. (2011) Knockout of G protein ?5 impairs brain development and causes multiple neurologic abnormalities in mice. J Neurochem 119:544-54|
|Panicker, Leelamma M; Zhang, Jian-Hua; Posokhova, Ekaterina et al. (2010) Nuclear localization of the G protein beta 5/R7-regulator of G protein signaling protein complex is dependent on R7 binding protein. J Neurochem 113:1101-12|