This proposal seeks to test the hypothesis that the myocardial sarcoplasmic reticulum undergoes profound changes during the transition from stable compensatory hypertrophy to decompensation, dilatation, and failure. Furthermore, these changes, which affect the release process(es) as well as kinetics of unidirectional calcium uptake, are caused by intrinsic changes in the sarcoplasmic reticulum as well as regulatory systems involving calmodulin, cAMP, and other second messengers. This hypothesis will be tested using a strain of hypertensive, obese, male rats with non-insulin-dependent diabetes mellitus, the SHHF/Mcc-cp. These animals all develop a fatal dilated cardiomyopathy between 10 and 12 months of age. Contractile performance, with special emphasis on mechanical alternans and the interval-strength relationship, will be investigated in Langendorff perfused SHHF hearts at various stages during the transition to failure. Simultaneous 31P-NMR spectra will be obtained to assess energy status and intracellular pH. Ventricular myocytes will be isolated from SHHF hearts at varying stages during the transition to failure and characterized in terms of electrically-stimulated Ca-dependent fura-2 ratio transients, hormone responsiveness, membrane receptors, and cAMP and IP 3 production. Calmodulin and proteins of the sarcoplasmic reticulum including calsequestrin, phospholamban, the ryanodine-binding release channel protein, and Ca-dependent ATPase will also be quantified in intact myocytes. Lysed myocytes will be used to probe 45Ca 2+ uptake and release by the physically intact, in situ, sarcoplasmic reticulum. Results will be compared to those from age-matched control WKY/N and estrone pre-treated obese SHHF male rats. The latter develop end-stage failure several months later than untreated obese males.
Showing the most recent 10 out of 32 publications
