1) We are continuing our work on exosomes as a source of biomarkers, including methods to improve recovery of exosomal proteins and miRNA. 2) We have also entered into a public-private consortium with the HESI Genomics microRNA Focus Group to compare standard protocol vs site-specific protocols from multiple sites;variations will identify which preanalytical steps affect microRNA detection and quantification in biofluids in drug-induced injury models. The initial model is a cardiotoxin that stimulates release of miRNAs into the circulation, which will shed light on renal handling of miRNA. Urinary miRNA from non-renal sources is expected to be non-exosomal. 3) We continue to work on alternatives to serum creatinine as surrogates for glomuerular filtration rate, such as serum cystatin C.

Project Start
Project End
Budget Start
Budget End
Support Year
13
Fiscal Year
2012
Total Cost
$562,073
Indirect Cost
City
State
Country
Zip Code
Cai, Yan; Xu, Ming-Jiang; Koritzinsky, Erik H et al. (2017) Mitochondrial DNA-enriched microparticles promote acute-on-chronic alcoholic neutrophilia and hepatotoxicity. JCI Insight 2:
Street, J M; Koritzinsky, E H; Glispie, D M et al. (2017) Urine Exosomes: An Emerging Trove of Biomarkers. Adv Clin Chem 78:103-122
Koritzinsky, Erik H; Street, Jonathan M; Star, Robert A et al. (2017) Quantification of Exosomes. J Cell Physiol 232:1587-1590
Street, Jonathan M; Koritzinsky, Erik H; Glispie, Deonna M et al. (2017) Urine Exosome Isolation and Characterization. Methods Mol Biol 1641:413-423
Koritzinsky, Erik H; Street, Jonathan M; Star, Robert A et al. (2017) Cover Image, Volume 232, Number 7, July 2017. J Cell Physiol 232:i
Souza, Ana Carolina P; Bocharov, Alexander V; Baranova, Irina N et al. (2016) Antagonism of scavenger receptor CD36 by 5A peptide prevents chronic kidney disease progression in mice independent of blood pressure regulation. Kidney Int 89:809-22
Askenazi, David J; Morgan, Catherine; Goldstein, Stuart L et al. (2016) Strategies to improve the understanding of long-term renal consequences after neonatal acute kidney injury. Pediatr Res 79:502-8
Thompson, Karol L; Boitier, Eric; Chen, Tao et al. (2016) Absolute Measurement of Cardiac Injury-Induced microRNAs in Biofluids across Multiple Test Sites. Toxicol Sci 154:115-125
Bavendam, Tamara G; Norton, Jenna M; Kirkali, Ziya et al. (2016) Advancing a Comprehensive Approach to the Study of Lower Urinary Tract Symptoms. J Urol 196:1342-1349
Leelahavanichkul, Asada; Souza, Ana Carolina P; Street, Jonathan M et al. (2014) Comparison of serum creatinine and serum cystatin C as biomarkers to detect sepsis-induced acute kidney injury and to predict mortality in CD-1 mice. Am J Physiol Renal Physiol 307:F939-48

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