1) We are continuing our work on exosomes as a source of biomarkers, including methods to improve recovery of exosomal proteins and miRNA. 2) We have also entered into a public-private consortium with the HESI Genomics microRNA Focus Group to compare standard protocol vs site-specific protocols from multiple sites;variations will identify which preanalytical steps affect microRNA detection and quantification in biofluids in drug-induced injury models. The initial model is a cardiotoxin that stimulates release of miRNAs into the circulation, which will shed light on renal handling of miRNA. Urinary miRNA from non-renal sources is expected to be non-exosomal. 3) We continue to work on alternatives to serum creatinine as surrogates for glomuerular filtration rate, such as serum cystatin C.

Project Start
Project End
Budget Start
Budget End
Support Year
13
Fiscal Year
2012
Total Cost
$562,073
Indirect Cost
City
State
Country
Zip Code
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