1) We are continuing our work on exosomes as a source of biomarkers, including proteins and miRNA. 2) We are continuing a public-private consortium with the HESI Genomics microRNA Focus Group to compare standard protocol vs site-specific protocols from multiple sites;variations will identify which preanalytical steps affect microRNA detection and quantification in biofluids in drug-induced injury models. The initial model is a cardiotoxin that stimulates release of miRNAs into the circulation, which will shed light on renal handling of miRNA. Urinary miRNA from non-renal sources is expected to be non-exosomal. 3) We continue to work on alternatives to serum creatinine as surrogates for glomuerular filtration rate, such as serum cystatin C.

Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Zip Code
Street, Jonathan M; Souza, Ana Carolina P; Alvarez-Prats, Alejandro et al. (2014) Automated quantification of renal fibrosis with Sirius Red and polarization contrast microscopy. Physiol Rep 2:
Street, Jonathan M; Yuen, Peter S T; Star, Robert A (2014) Bioactive exosomes: possibilities for diagnosis and management of bladder cancer. J Urol 192:297-8
Zhou, Hua; Kajiyama, Hiroshi; Tsuji, Takayuki et al. (2013) Urinary exosomal Wilms' tumor-1 as a potential biomarker for podocyte injury. Am J Physiol Renal Physiol 305:F553-9
Michael, A; Bajracharya, S D; Yuen, P S T et al. (2010) Exosomes from human saliva as a source of microRNA biomarkers. Oral Dis 16:34-8
Doi, Kent; Yuen, Peter S T; Eisner, Christoph et al. (2009) Reduced production of creatinine limits its use as marker of kidney injury in sepsis. J Am Soc Nephrol 20:1217-21
Gonzales, Patricia A; Pisitkun, Trairak; Hoffert, Jason D et al. (2009) Large-scale proteomics and phosphoproteomics of urinary exosomes. J Am Soc Nephrol 20:363-79
Hewitt, Stephen M; Star, Robert A (2008) Frozen protein arrays. Methods Mol Biol 441:105-12
Mullins, Chris; Lucia, M Scott; Hayward, Simon W et al. (2008) A comprehensive approach toward novel serum biomarkers for benign prostatic hyperplasia: the MPSA Consortium. J Urol 179:1243-56