In collaboration with Dr. Michael Tarlov, we have further investigated the optimal sensing layer design for the characterization of the carbohydrate moiety of glycoproteins. This resulted in the recognition that multi-valency of lectins is an important factor in the choice of immobilization strategy. Previously we had recognized surface site heterogeneity is a key problem of surface binding analysis, and developed a method for extracting affinity and kinetic rate distributions from experimental data. For this purpose we had programmed a software tool that is now widely used. We have now further improved this program by facilitating the application of corrections for mass transport limited binding, broadening the applicability of this approach.
|Zhao, Huaying; Gorshkova, Inna I; Fu, Gregory L et al. (2013) A comparison of binding surfaces for SPR biosensing using an antibody-antigen system and affinity distribution analysis. Methods 59:328-35|
|Zhao, Huaying; Brown, Patrick H; Schuck, Peter (2011) On the distribution of protein refractive index increments. Biophys J 100:2309-17|
|Schuck, Peter; Zhao, Huaying (2010) The role of mass transport limitation and surface heterogeneity in the biophysical characterization of macromolecular binding processes by SPR biosensing. Methods Mol Biol 627:15-54|
|Gondeau, Claire; Corradin, Giampietro; Heitz, Frédéric et al. (2009) The C-terminal domain of Plasmodium falciparum merozoite surface protein 3 self-assembles into alpha-helical coiled coil tetramer. Mol Biochem Parasitol 165:153-61|