The Imaging Core Unit (ICU) provides NEI intramural scientists access and training in a variety of high resolution imaging and analysis applications including confocal microscopy (Leica SP2 AOBS and Zeiss 700 laser scanning confocal microscope), total internal reflectance fluorescence ( Zeiss -TIRF), laser capture microdissection (Zeiss-PALM), ex vivo imaging of retina explants and Forster resonance energy transfer (FRET). A new 2-photon microscope (Olympus Fluoview 1000 + Spectra Physics Mai Tai Deep See TiSapphire laser) has dramatically expand opportunities for imaging ocular tissues at greater depths and with reduced photodamage. A primary objective of the ICU is to pair state-of-the-art instrumentation with novel imaging approaches in order to leverage studies with the potential for significant clinical application. The ICU has developed improved methods for quantifying choroidal neovascularization in an experimental animal model. These studies have in turn lead to the identification of potent anti-angiogenic agents with the potential for retarding new blood vessel growth associated with the wet form of age-related macular degeneration.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Scientific Cores Intramural Research (ZIC)
Project #
1ZICEY000459-06
Application #
8737696
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
2013
Total Cost
$589,256
Indirect Cost
Name
U.S. National Eye Institute
Department
Type
DUNS #
City
State
Country
Zip Code
Li, Yichao; Fariss, Robert N; Qian, Jennifer W et al. (2016) Light-Induced Thickening of Photoreceptor Outer Segment Layer Detected by Ultra-High Resolution OCT Imaging. Invest Ophthalmol Vis Sci 57:OCT105-11
Tam, Johnny; Liu, Jianfei; Dubra, Alfredo et al. (2016) In Vivo Imaging of the Human Retinal Pigment Epithelial Mosaic Using Adaptive Optics Enhanced Indocyanine Green Ophthalmoscopy. Invest Ophthalmol Vis Sci 57:4376-84
Wang, Xu; Zhao, Lian; Zhang, Jun et al. (2016) Requirement for Microglia for the Maintenance of Synaptic Function and Integrity in the Mature Retina. J Neurosci 36:2827-42
Zabel, Matthew K; Zhao, Lian; Zhang, Yikui et al. (2016) Microglial phagocytosis and activation underlying photoreceptor degeneration is regulated by CX3CL1-CX3CR1 signaling in a mouse model of retinitis pigmentosa. Glia 64:1479-91
Indaram, Maanasa; Ma, Wenxin; Zhao, Lian et al. (2015) 7-Ketocholesterol increases retinal microglial migration, activation, and angiogenicity: a potential pathogenic mechanism underlying age-related macular degeneration. Sci Rep 5:9144
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Zhao, Lian; Zabel, Matthew K; Wang, Xu et al. (2015) Microglial phagocytosis of living photoreceptors contributes to inherited retinal degeneration. EMBO Mol Med 7:1179-97
Rachel, Rivka A; Yamamoto, Erin A; Dewanjee, Mrinal K et al. (2015) CEP290 alleles in mice disrupt tissue-specific cilia biogenesis and recapitulate features of syndromic ciliopathies. Hum Mol Genet 24:3775-91
Fritsche, Lars G; Fariss, Robert N; Stambolian, Dwight et al. (2014) Age-related macular degeneration: genetics and biology coming together. Annu Rev Genomics Hum Genet 15:151-71
Kim, Soo-Young; Yang, Hyun-Jin; Chang, Yi-Sheng et al. (2014) Deletion of Aryl Hydrocarbon Receptor AHR in Mice Leads to Subretinal Accumulation of Microglia and RPE Atrophy. Invest Ophthalmol Vis Sci 55:6031-40

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