Funding Agency

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
NIH Director’s Pioneer Award (NDPA) (DP1)
Project #
5DP1OD000122-02
Application #
6953719
Study Section
Special Emphasis Panel (ZRG1-NDPA (50))
Program Officer
Wilder, Elizabeth L
Project Start
2004-09-30
Project End
2009-07-31
Budget Start
2005-08-01
Budget End
2006-07-31
Support Year
2
Fiscal Year
2005
Total Cost
$770,000
Indirect Cost

  Institution

Name
Duke University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
044387793
City
Durham
State
NC
Country
United States
Zip Code
27705

  Publications

Grimley, Joshua S; Li, Li; Wang, Weina et al. (2013) Visualization of synaptic inhibition with an optogenetic sensor developed by cell-free protein engineering automation. J Neurosci 33:16297-309
Layton, Curtis J; Hellinga, Homme W (2011) Integration of cell-free protein coexpression with an enzyme-linked immunosorbent assay enables rapid analysis of protein-protein interactions directly from DNA. Protein Sci 20:1432-8
Isom, Daniel G; Marguet, Philippe R; Oas, Terrence G et al. (2011) A miniaturized technique for assessing protein thermodynamics and function using fast determination of quantitative cysteine reactivity. Proteins 79:1034-47
Allert, Malin; Cox, J Colin; Hellinga, Homme W (2010) Multifactorial determinants of protein expression in prokaryotic open reading frames. J Mol Biol 402:905-18
Isom, Daniel G; Vardy, Eyal; Oas, Terrence G et al. (2010) Picomole-scale characterization of protein stability and function by quantitative cysteine reactivity. Proc Natl Acad Sci U S A 107:4908-13
Layton, Curtis J; Hellinga, Homme W (2010) Thermodynamic analysis of ligand-induced changes in protein thermal unfolding applied to high-throughput determination of ligand affinities with extrinsic fluorescent dyes. Biochemistry 49:10831-41
Cuneo, Matthew J; Beese, Lorena S; Hellinga, Homme W (2009) Structural analysis of semi-specific oligosaccharide recognition by a cellulose-binding protein of thermotoga maritima reveals adaptations for functional diversification of the oligopeptide periplasmic binding protein fold. J Biol Chem 284:33217-23
Cuneo, Matthew J; Changela, Anita; Beese, Lorena S et al. (2009) Structural adaptations that modulate monosaccharide, disaccharide, and trisaccharide specificities in periplasmic maltose-binding proteins. J Mol Biol 389:157-66
Allert, Malin; Dwyer, Mary A; Hellinga, Homme W (2007) Local encoding of computationally designed enzyme activity. J Mol Biol 366:945-53
Rizk, Shahir S; Cuneo, Matthew J; Hellinga, Homme W (2006) Identification of cognate ligands for the Escherichia coli phnD protein product and engineering of a reagentless fluorescent biosensor for phosphonates. Protein Sci 15:1745-51

Showing the most recent 10 out of 11 publications