In the fields of biomaterials and tissue engineering, the ability to modulate cell behavior on surfaces is essential, particularly when attempting to direct cell growth and differentiation, both for in vitro engineered tissue and generating cell sources from progenitors and/or stem cells. A major area of work in tissue engineering is the development of artificial extracellular matrices or scaffolds. The extracellular in vivo is a dynamic entity that often shifts between a composition of one distinct set of components to another. This matrix remodeling is especially common during development, differentiation, and wound repair. A dynamic engineered matrix that emulates this situation in vitro has yet to be developed and would have a huge impact in tissue engineering and regenerative medicine. For example, one of the biggest challenges to realizing stem cell therapies is the ability to take a premature cell and differentiate it into the desired phenotype. While soluble factors and static matrices have been examined for accomplishing this goal, the technology that could mimic the developing extracellular matrix, which is known to regulate cell survival, migration, proliferation, and differentiation, has not been developed. The principal investigator aims to create such a dynamic matrix microenvironment that could promote differentiation by mimicking extracellular matrix morphogenesis. Therefore, differentiated cell sources from progenitors and stem cells could be more efficiently obtained, which would have direct impact on promoting and advancing cell therapies, including engineered tissue. This work will be enabled by a novel and innovative, multi-layer format patterning technique developed by the principal investigator.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
NIH Director’s New Innovator Awards (DP2)
Project #
3DP2OD004309-01S1
Application #
7892255
Study Section
Special Emphasis Panel (ZGM1-NDIA-G (01))
Program Officer
Basavappa, Ravi
Project Start
2009-08-10
Project End
2011-09-30
Budget Start
2009-08-10
Budget End
2011-09-30
Support Year
1
Fiscal Year
2009
Total Cost
$41,973
Indirect Cost
Name
University of California San Diego
Department
Engineering (All Types)
Type
Schools of Arts and Sciences
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Adam Young, D; Bajaj, Vaibhav; Christman, Karen L (2014) Award winner for outstanding research in the PhD category, 2014 Society for Biomaterials annual meeting and exposition, Denver, Colorado, April 16-19, 2014: Decellularized adipose matrix hydrogels stimulate in vivo neovascularization and adipose formation J Biomed Mater Res A 102:1641-51
Rao, Nikhil; Grover, Gregory N; Vincent, Ludovic G et al. (2013) A co-culture device with a tunable stiffness to understand combinatorial cell-cell and cell-matrix interactions. Integr Biol (Camb) 5:1344-54
Grover, Gregory N; Braden, Rebecca L; Christman, Karen L (2013) Oxime Cross-Linked Injectable Hydrogels for Catheter Delivery. Adv Mater :
Young, D Adam; Choi, Yu Suk; Engler, Adam J et al. (2013) Stimulation of adipogenesis of adult adipose-derived stem cells using substrates that mimic the stiffness of adipose tissue. Biomaterials 34:8581-8
Rao, Nikhil; Evans, Samantha; Stewart, Danique et al. (2013) Fibroblasts influence muscle progenitor differentiation and alignment in contact independent and dependent manners in organized co-culture devices. Biomed Microdevices 15:161-9
DeQuach, Jessica A; Lin, Joy E; Cam, Cynthia et al. (2012) Injectable skeletal muscle matrix hydrogel promotes neovascularization and muscle cell infiltration in a hindlimb ischemia model. Eur Cell Mater 23:400-12; discussion 412
Young, D A; Christman, K L (2012) Injectable biomaterials for adipose tissue engineering. Biomed Mater 7:024104
Seif-Naraghi, Sonya B; Horn, Dinah; Schup-Magoffin, Pamela J et al. (2012) Injectable extracellular matrix derived hydrogel provides a platform for enhanced retention and delivery of a heparin-binding growth factor. Acta Biomater 8:3695-703
Song, Airong; Rane, Aboli A; Christman, Karen L (2012) Antibacterial and cell-adhesive polypeptide and poly(ethylene glycol) hydrogel as a potential scaffold for wound healing. Acta Biomater 8:41-50
Singelyn, Jennifer M; Sundaramurthy, Priya; Johnson, Todd D et al. (2012) Catheter-deliverable hydrogel derived from decellularized ventricular extracellular matrix increases endogenous cardiomyocytes and preserves cardiac function post-myocardial infarction. J Am Coll Cardiol 59:751-63

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